Anaplasmosis is one of the most prevalent tick-borne diseases of cattle caused by Anaplasma marginale. MSP1a surface protein has been shown to be involved in eliciting immunity to infected cattle. Carbon nanotubes (CNTs) has been increasingly highlighted due to their needle like structure, which contain multiple attachment sites for biomolecules and may interact with or cross biological membranes, increasing antigen availability to immune system. Here, we have successfully designed a nanocomplex of a synthetic peptide noncovalently attached to multiwalled CNT (MWCNT). Peptide comprising the core motif of the MSP1a was efficiently adsorb onto the nanoparticle surface. The MWCNT-Am1 nanocomplex exhibited high stability and good dispersibility and in vivo immunization showed high levels of IgG1 and IgG2a, followed by increased expression of pro-inflammatory and anti-inflammatory cytokines. This is a proof-of-concept of a nanovaccine that was able to generate a strong immune response compared to the common antigen-adjuvant vaccine without the nanoparticles.

中文翻译：

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MSP1a功能性基序偶联多壁碳纳米管诱导的平衡Th1 / Th2免疫应答作为鼠模型中的抗厌氧菌疫苗。

无性病是由无性无形体引起的最常见的tick传播疾病之一。MSP1a表面蛋白已显示参与引起对感染牛的免疫力。碳纳米管（CNTs）由于其针状结构而日益受到关注，该结构包含多个生物分子附着位点，并可能与生物膜相互作用或穿越生物膜，从而增加了免疫系统的抗原利用率。在这里，我们已经成功设计了非共价连接到多壁CNT（MWCNT）的合成肽的纳米复合物。包含MSP1a核心图案的肽可有效吸附到纳米颗粒表面。MWCNT-Am1纳米复合物显示出高稳定性和良好的分散性，体内免疫显示出高水平的IgG1和IgG2a，然后增加促炎和抗炎细胞因子的表达。这是纳米疫苗的概念证明，与没有纳米颗粒的普通抗原佐剂疫苗相比，它能够产生强大的免疫反应。