Evidence of the gut microbiota influencing neurodegenerative diseases has been reported for several neural diseases. However, there is little insight regarding the relationship between the gut microbiota and prion disease. Here, using fecal samples of 12 prion-infected mice and 25 healthy controls, we analyzed the structure of the gut microbiota and metabolic changes by 16S rRNA sequencing and LC-MS-based metabolomics respectively as multi-omic analyses. Additionally, SCFAs and common amino acids were detected by GC-MS and UPLC respectively. Enteric changes induced by prion disease affected both structure and abundances of the gut microbiota. The gut microbiota of infected mice displayed greater numbers of Proteobacteria and less Saccharibacteria at the phylum level and more Lactobacillaceae and Helicobacteraceae and less Prevotellaceae and Ruminococcaceae at the family level. A total of 145 fecal metabolites were found to be significantly different in prion infection, and most (114) of these were lipid metabolites. Using KEGG pathway enrichment analysis, we found that 3 phosphatidylcholine (PC) compounds significantly decreased and 4 hydrophobic bile acids significantly increased. Decreases of 8 types of short-chain acids (SCFAs) and increases of Cys and Tyr and decreases of His, Trp, and Arg were observed in prion infection. Correlation analysis indicated that the gut microbiota changes observed in our study may have been the shared outcome of prion disease. These findings suggest that prion disease can cause significant shifts in the gut microbiota. Certain bacterial taxa can then respond to the resulting change to the enteric environment by causing dramatic shifts in metabolite levels. Our data highlight the health impact of the gut microbiota and related metabolites in prion disease.

中文翻译：

---

肠道微生物群失调和and病毒疾病代谢变化的影响。

已经报道了几种微生物疾病影响肠道微生物群影响神经退行性疾病的证据。然而，关于肠道菌群与病毒疾病之间关系的见解很少。在这里，我们使用12只samples病毒感染的小鼠和25位健康对照的粪便样本，分别通过16S rRNA测序和基于LC-MS的代谢组学分析了肠道菌群的结构和代谢变化，作为多组学分析。此外，分别通过GC-MS和UPLC检测了SCFA和常见氨基酸。由病毒病引起的肠变化影响肠道菌群的结构和丰度。受感染小鼠的肠道菌群在门类水平上表现出更多的变形杆菌数量和更少的糖细菌，在家庭水平上表现出更多的乳酸杆菌科和幽门螺杆菌以及更少的前卫纲和Ruminococcaceae。发现总共145种粪便代谢产物在病毒感染中有显着差异，其中大多数（114）是脂质代谢产物。使用KEGG途径富集分析，我们发现3种磷脂酰胆碱（PC）化合物显着减少，而4种疏水性胆汁酸显着增加。在病毒感染中观察到8种类型的短链酸（SCFA）减少，Cys和Tyr升高，His，Trp和Arg降低。相关分析表明，在我们的研究中观察到的肠道菌群变化可能是病毒病的共同结果。这些发现表明病毒病可以引起肠道菌群的显着变化。然后，某些细菌类群可以通过引起代谢物水平的急剧变化来响应肠道环境的最终变化。我们的数据突出了肠道微生物群和相关代谢产物对病毒疾病的健康影响。