Drosophila RpS12 controls translation, growth, and cell competition through Xrp1.,PLOS Genetics

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Drosophila RpS12 controls translation, growth, and cell competition through Xrp1.
PLOS Genetics ( IF 4.0 ) Pub Date : 2019-12-16 , DOI: 10.1371/journal.pgen.1008513
Zhejun Ji ₁ , Marianthi Kiparaki ₁ , Virginia Folgado ₁ , Amit Kumar ₁ , Jorge Blanco ₁ , Gerard Rimesso ₁ , Jacky Chuen ₁ , Yang Liu ₁ , Deyou Zheng _{1,

2,

3} , Nicholas E Baker _{1,

4,

5}

Affiliation

Whereas complete loss of Rp function is generally lethal, most heterozygous Rp mutants grow more slowly and are subject to competitive loss from mosaics tissues that also contain wild type cells. The rpS12 gene has a special role in the cell competition of other Ribosomal Protein (Rp) mutant cells in Drosophila. Elimination by cell competition is promoted by higher RpS12 levels and prevented by a specific rpS12 mis-sense mutation, identifying RpS12 as a key effector of cell competition due to mutations in other Rp genes. Here we show that RpS12 is also required for other aspects of Rp mutant phenotypes, including hundreds of gene expression changes that occur in 'Minute' Rp heterozygous wing imaginal discs, overall translation rate, and the overall rate of organismal development, all through the bZip protein Xrp1 that is one of the RpS12-regulated genes. Our findings outline the regulatory response to mutations affecting essential Rp genes that controls overall translation, growth, and cell competition, and which may contribute to cancer and other diseases.

中文翻译：

果蝇RpS12通过Xrp1控制翻译，生长和细胞竞争。

尽管Rp功能的完全丧失通常是致命的，但大多数杂合Rp突变体的生长速度较慢，并且会从也包含野生型细胞的镶嵌组织中遭受竞争性丧失。rpS12基因在果蝇中其他核糖体蛋白（Rp）突变细胞的细胞竞争中具有特殊作用。较高的RpS12水平促进了细胞竞争的消除，并且通过特定的rpS12错义突变得以阻止，从而将RpS12鉴定为由于其他Rp基因的突变而引起的细胞竞争的关键效应器。在这里，我们显示Rp突变表型的其他方面也需要RpS12，包括在'Minute'Rp杂合子翼假想盘中发生的数百种基因表达变化，总体翻译率以及生物体发育的总体速度，全部通过bZip蛋白Xrp1进行，该蛋白是RpS12调控的基因之一。我们的发现概述了对影响必需Rp基因的突变的调控反应，该基因控制总体翻译，生长和细胞竞争，并可能导致癌症和其他疾病。

更新日期：2019-12-17

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