Background/aims The dysregulation of circABCB10 may play an critical role in tumor progression. However, its function in liver cancer (HCC) is still unclear. Therefore, this experimental design is based on circABCB10 to explore the pathogenesis of HCC. Methods The expression of circABCB10 and miR-670-3p in HCC tissues was detected by RT-qPCR. CCK-8, Brdu incorporation, colony formation and transwell assays were used to determine the effect of circABCB10 on HCC cell proliferation and migration. Target gene prediction and screening, luciferase reporter assays were used to validate downstream target genes of circABCB10 and miR-670-3p. HMG20A expression was detected by RT-qPCR and Western blotting. The tumor changes in mice were detected by in nude mice. Results CircABCB10 was significantly increased in HCC tissues and cell lines, and high CircABCB10 expression was directly associated with low survival in HCC patients. Silencing of circABCB10 inhibited proliferation and invasion of hepatocellular carcinoma. In addition, circABCB10 acted as a sponge of miR-670-3p to upregulate HMG20A expression. In addition, overexpression of miR-670-3p or knockdown of HMG20A reversed the carcinogenic effects of circABCB10 in HCC. There was a negative correlation between the expression of circABCB10 and miR-670-3p, and a positive correlation between the expression of circABCB10 and HMG20A in HCC tissues. Conclusion circABCB10 promoted HCC progression by modulating the miR-670-3p/HMG20A axis, and circABCB10 may be a potential therapeutic target for HCC.Trail registration JL1H384739, registered at Sep 09, 2014.

中文翻译：

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环状 RNA ABCB10 通过海绵化 miR-670-3p 增加 HMG20A 的表达来促进肝细胞癌的进展。

背景/目的 circABCB10 的失调可能在肿瘤进展中起关键作用。然而，其在肝癌（HCC）中的作用仍不清楚。因此，本实验设计基于circABCB10来探索HCC的发病机制。方法 RT-qPCR检测HCC组织中circABCB10和miR-670-3p的表达。CCK-8、Brdu 掺入、集落形成和 transwell 测定用于确定 circABCB10 对 HCC 细胞增殖和迁移的影响。靶基因预测和筛选，荧光素酶报告基因检测用于验证 circABCB10 和 miR-670-3p 的下游靶基因。通过RT-qPCR和Western印迹检测HMG20A的表达。在裸鼠中检测到小鼠的肿瘤变化。结果 CircABCB10 在 HCC 组织和细胞系中显着升高，高 CircABCB10 表达与 HCC 患者的低生存率直接相关。circABCB10的沉默抑制了肝癌的增殖和侵袭。此外，circABCB10 作为 miR-670-3p 的海绵上调 HMG20A 表达。此外，过表达 miR-670-3p 或敲低 HMG20A 可逆转 circABCB10 在 HCC 中的致癌作用。HCC组织中circABCB10和miR-670-3p的表达呈负相关，circABCB10和HMG20A的表达呈正相关。结论 circABCB10通过调节miR-670-3p/HMG20A轴促进HCC进展，circABCB10可能是HCC的潜在治疗靶点。跟踪注册JL1H384739，注册于2014年9月9日。circABCB10的沉默抑制了肝癌的增殖和侵袭。此外，circABCB10 作为 miR-670-3p 的海绵上调 HMG20A 表达。此外，过表达 miR-670-3p 或敲低 HMG20A 可逆转 circABCB10 在 HCC 中的致癌作用。HCC组织中circABCB10和miR-670-3p的表达呈负相关，circABCB10和HMG20A的表达呈正相关。结论 circABCB10通过调节miR-670-3p/HMG20A轴促进HCC进展，circABCB10可能是HCC的潜在治疗靶点。跟踪注册JL1H384739，注册于2014年9月9日。circABCB10的沉默抑制了肝癌的增殖和侵袭。此外，circABCB10 作为 miR-670-3p 的海绵上调 HMG20A 表达。此外，过表达 miR-670-3p 或敲低 HMG20A 可逆转 circABCB10 在 HCC 中的致癌作用。HCC组织中circABCB10和miR-670-3p的表达呈负相关，circABCB10和HMG20A的表达呈正相关。结论 circABCB10通过调节miR-670-3p/HMG20A轴促进HCC进展，circABCB10可能是HCC的潜在治疗靶点。跟踪注册JL1H384739，注册于2014年9月9日。此外，过表达 miR-670-3p 或敲低 HMG20A 可逆转 circABCB10 在 HCC 中的致癌作用。HCC组织中circABCB10和miR-670-3p的表达呈负相关，circABCB10和HMG20A的表达呈正相关。结论 circABCB10通过调节miR-670-3p/HMG20A轴促进HCC进展，circABCB10可能是HCC的潜在治疗靶点。跟踪注册JL1H384739，注册于2014年9月9日。此外，过表达 miR-670-3p 或敲低 HMG20A 可逆转 circABCB10 在 HCC 中的致癌作用。HCC组织中circABCB10和miR-670-3p的表达呈负相关，circABCB10和HMG20A的表达呈正相关。结论 circABCB10通过调节miR-670-3p/HMG20A轴促进HCC进展，circABCB10可能是HCC的潜在治疗靶点。跟踪注册JL1H384739，注册于2014年9月9日。