Background Ovarian cancer is one of the leading causes of female death worldwide, with a poor prognosis of advanced patients. Sevoflurane, a volatile anesthetic commonly used in clinical operations, has been reported to have anti-cancer activity against some tumors. In the present study, we aimed to investigate the effects of sevoflurane on the progression of ovarian cancer and its potential mechanism. Methods The effects of sevoflurane on ovarian cancer cell viability, proliferation, migration, invasion, cell cycle, and apoptosis were determined by functional experiments in vitro. Gelatin zymography assay was performed to examine MMP9 activity. In vivo, sevoflurane was injected into mice of transplantation tumor with SKOV3 cells or with pcDNA-STC1 treated SKOV3 cells. Results We found that sevoflurane inhibited the viability of SKOV3 and OVCAR3 cells in a dose-dependent manner, and colony formation assay revealed that sevoflurane inhibited ovarian cancer cell colony-formation abilities. Additionally, sevoflurane could induce cell cycle arrest and promote cell apoptosis in SKOV3 and OVCAR3 cells. Moreover, sevoflurane reduced the migration and invasion abilities of SKOV3 and OVCAR3 cells, as well as the MMP-9 activity. Furthermore, sevoflurane down-regulated the expression of stanniocalcin 1 (STC1), and up-regulation of STC1 could reverse the inhibitory effects of sevoflurane on cell proliferation and invasion. In vivo, sevoflurane significantly inhibited the tumor growth, which was be reversed by STC1 overexpression. Conclusion These data reveal an anti-cancer activity of sevoflurane on the growth and invasion of ovarian cancer, which may be through down-regulating STC1. Sevoflurane may serve as a potential anti-cancer agent in ovarian cancer therapy.

中文翻译：

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七氟醚通过下调斯钙素 1 (STC1) 抑制卵巢癌的进展。

背景 卵巢癌是全球女性死亡的主要原因之一，晚期患者预后较差。七氟醚是一种常用于临床手术的挥发性麻醉剂，据报道对某些肿瘤具有抗癌活性。本研究旨在探讨七氟醚对卵巢癌进展的影响及其潜在机制。方法通过体外功能实验，测定七氟醚对卵巢癌细胞活力、增殖、迁移、侵袭、细胞周期和凋亡的影响。进行明胶酶谱测定以检查MMP9活性。在体内，将七氟醚注射到具有 SKOV3 细胞或 pcDNA-STC1 处理的 SKOV3 细胞的移植肿瘤小鼠中。结果我们发现七氟醚以剂量依赖性方式抑制SKOV3和OVCAR3细胞的活力，集落形成试验显示七氟醚抑制卵巢癌细胞集落形成能力。此外，七氟醚可在 SKOV3 和 OVCAR3 细胞中诱导细胞周期停滞并促进细胞凋亡。此外，七氟醚降低了 SKOV3 和 OVCAR3 细胞的迁移和侵袭能力，以及 MMP-9 的活性。此外，七氟醚下调斯钙素1（STC1）的表达，而上调STC1可以逆转七氟醚对细胞增殖和侵袭的抑制作用。在体内，七氟醚显着抑制肿瘤生长，而 STC1 过表达可逆转肿瘤生长。结论 这些数据揭示了七氟醚对卵巢癌生长和侵袭的抗癌活性，这可能是通过下调STC1来实现的。七氟醚可作为卵巢癌治疗的潜在抗癌剂。