BACKGROUND Peste des petits ruminants (PPR) is a viral disease of major economic importance on small ruminants. Goats are usually known to be more susceptible to the disease. Infection chronology, virus circulation, and the disease early detection need to be better understood. This study evaluates the tissue tropism and pathogenesis of PPR following experimental infection of goats using a lineage IV virus, the most dominant in the world originated from Asia. PPRV infection was experimentally induced in 4 six-month-old goats by intra-nasal and intravenous route of cell virus suspension and from infectious mashed tissue. The clinical signs were observed and goats were euthanized at predetermined clinical score level for post-mortem examinations and PPRV detection by RT-PCR. Clinical signs of infection were present, pyrexia, serous-mucopurulent nasal discharges, coughing, diarrhea and asthenia, for both cell virus suspension and infectious mashed tissue. PPRV genome was highly detected in swabs and tissues with clinical signs dominated by pulmonary attack and digestive symptoms secondary. RESULTS Results of this study indicates that PPRV is an invasive infection in animals that in a short period, less than 10 days, invade all vital organs. On live animals, early diagnostic may be easily done on lacrimal and rectal swabs. CONCLUSION The experimental PPRV-infection model using the cell virus suspension is suitable for vaccine evaluation as a standard model.

中文翻译：

---

摩洛哥2015年分离株对实验性感染山羊的小反刍动物发病机理的研究。

背景技术小反刍兽疫（PPR）是一种对小反刍动物具有重要经济意义的病毒性疾病。通常已知山羊对这种疾病更易感。感染年表，病毒传播和疾病的早期发现需要得到更好的了解。这项研究评估了使用世袭IV病毒对山羊进行实验性感染后PPR的组织嗜性和发病机理，这是世界上最主要的起源于亚洲的IV病毒。PPRV感染是通过鼻内和静脉内细胞病毒悬浮液以及感染性捣烂组织在4只6个月大的山羊中通过实验诱导的。观察到临床体征，并以预定的临床评分水平对安乐死的山羊进行死后检查和RT-PCR检测PPRV。存在感染的临床体征，发热，浆液性粘液性鼻分泌物，咳嗽，腹泻和乏力，用于细胞病毒悬浮液和传染性捣碎组织。在拭子和组织中高度检测到PPRV基因组，其临床症状主要为肺部攻击和继发的消化道症状。结果这项研究的结果表明，PPRV是动物的一种侵袭性感染，在短短不到10天的时间内即可侵袭所有重要器官。对于活体动物，很容易对泪腺和直肠拭子进行早期诊断。结论使用细胞病毒悬浮液的实验PPRV感染模型适合作为标准模型进行疫苗评估。在拭子和组织中高度检测到PPRV基因组，其临床症状主要为肺部攻击和继发的消化道症状。结果这项研究的结果表明，PPRV是动物的一种侵袭性感染，在短短不到10天的时间内即可侵袭所有重要器官。对于活体动物，很容易对泪腺和直肠拭子进行早期诊断。结论使用细胞病毒悬浮液的实验PPRV感染模型适合作为标准模型进行疫苗评估。在拭子和组织中高度检测到PPRV基因组，其临床体征主要由肺部发作和继发的消化系统症状引起。结果这项研究的结果表明，PPRV是动物的一种侵袭性感染，在短短不到10天的时间内即可侵袭所有重要器官。对于活体动物，很容易对泪腺和直肠拭子进行早期诊断。结论使用细胞病毒悬浮液的实验PPRV感染模型适合作为标准模型进行疫苗评估。