BACKGROUND Competitive endogenous RNAs (ceRNAs) have revealed a new mechanism of interaction between RNAs. However, an understanding of the ceRNA regulatory network in Wilms tumour (WT) remains limited. METHODS The expression profiles of mRNAs, miRNAs and lncRNAs in Wilms tumour samples and normal samples were obtained from the Therapeutically Applicable Research to Generate Effective Treatment (TARGET) database. The EdgeR package was employed to identify differentially expressed lncRNAs, miRNAs and mRNAs. Functional enrichment analyses via the ClusterProfile R package were performed, and the lncRNA-miRNA-mRNA interaction ceRNA network was established in Cytoscape. Subsequently, the correlation between the ceRNA network and overall survival was analysed. RESULTS A total of 2037 lncRNAs, 154 miRNAs and 3609 mRNAs were identified as differentially expressed RNAs in Wilms tumour. Of those, 205 lncRNAs, 26 miRNAs and 143 mRNAs were included in the ceRNA regulatory network. The results of Gene Ontology (GO) analysis revealed that the differentially expressed genes (DEGs) were mainly enriched in terms related to response to mechanical stimuli, transcription factor complexes, and transcription factor activity (related to RNA polymerase II proximal promoter sequence-specific DNA binding). The results of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the DEGs were mainly enriched in pathways related to the cell cycle. The survival analysis results showed that 16 out of the 205 lncRNAs, 1 out of 26 miRNAs and 5 out of 143 mRNAs were associated with overall survival in Wilms tumour patients (P < 0.05). CONCLUSIONS CeRNA networks play an important role in Wilms tumour. This finding might provide effective, novel insights for further understanding the mechanisms underlying Wilms tumour.

中文翻译：

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威尔姆斯肿瘤中lncRNA，miRNA和mRNA的竞争性内源RNA（ceRNA）调节网络。

背景技术竞争性内源RNA（ceRNA）揭示了RNA之间相互作用的新机制。然而，对Wilms肿瘤（WT）中的ceRNA调控网络的了解仍然有限。方法从治疗性应用研究产生有效治疗（TARGET）数据库中获得Wilms肿瘤样品和正常样品中mRNA，miRNA和lncRNA的表达谱。EdgeR软件包用于鉴定差异表达的lncRNA，miRNA和mRNA。通过ClusterProfile R软件包进行功能富集分析，并在Cytoscape中建立了lncRNA-miRNA-mRNA相互作用ceRNA网络。随后，分析了ceRNA网络与总体存活率之间的相关性。结果共有2037个lncRNA，在Wilms肿瘤中鉴定出154个miRNA和3609个mRNA为差异表达的RNA。其中有205个lncRNA，26个miRNA和143个mRNA包含在ceRNA调控网络中。基因本体论（GO）分析的结果表明，差异表达基因（DEG）主要在与对机械刺激，转录因子复合物和转录因子活性（与RNA聚合酶II近端启动子序列特异性DNA有关的应答）相关的方面富集捆绑）。京都基因与基因组百科全书（KEGG）途径分析的结果表明，DEG主要富集与细胞周期有关的途径。生存分析结果表明，205个lncRNA中的16个，26个miRNA中的1个和143个mRNA中的5个与Wilms肿瘤患者的总体生存相关（P <0.05）。结论CeRNA网络在Wilms肿瘤中起重要作用。这一发现可能为进一步了解威尔姆斯肿瘤的潜在机制提供有效，新颖的见解。