RNA sequencing using the latest single-molecule sequencing instruments produces reads that are thousands of nucleotides long. The ability to assemble these long reads can greatly improve the sensitivity of long-read analyses. Here we present StringTie2, a reference-guided transcriptome assembler that works with both short and long reads. StringTie2 includes new methods to handle the high error rate of long reads and offers the ability to work with full-length super-reads assembled from short reads, which further improves the quality of short-read assemblies. StringTie2 is more accurate and faster and uses less memory than all comparable short-read and long-read analysis tools.

中文翻译：

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使用 StringTie2 进行长读长 RNA-seq 比对的转录组组装

使用最新的单分子测序仪器进行 RNA 测序会产生数千个核苷酸长的读数。组装这些长读段的能力可以大大提高长读段分析的灵敏度。在这里，我们展示了 StringTie2，这是一个参考引导的转录组组装程序，可用于短读和长读。StringTie2 包括处理长读取高错误率的新方法，并提供处理由短读取组装的全长超级读取的能力，这进一步提高了短读取组装的质量。StringTie2 比所有类似的短读和长读分析工具更准确、更快，并且使用更少的内存。