BACKGROUND MCC/eisosomes are membrane microdomains that have been proposed to participate in the plasma membrane function in particular by regulating the homeostasis of lipids, promoting the recruitment of specific proteins and acting as provider of membrane reservoirs. RESULTS Here we showed that several potential MCC/eisosomal protein encoding genes in the necrotrophic fungus A. brassicicola were overexpressed when germinated spores were exposed to antimicrobial defence compounds, osmotic and hydric stresses, which are major constraints encountered by the fungus during the plant colonization process. Mutants deficient for key MCC/eisosome components did not exhibit any enhanced susceptibility to phytoalexins and to applied stress conditions compared to the reference strain, except for a slight hypersensitivity of the ∆∆abpil1a-abpil1b strain to 2 M sorbitol. Depending on the considered mutants, we showed that the leaf and silique colonization processes were impaired by comparison to the wild-type, and assumed that these defects in aggressiveness were probably caused by a reduced appressorium formation rate. CONCLUSIONS This is the first study on the role of MCC/eisosomes in the pathogenic process of a plant pathogenic fungus. A link between these membrane domains and the fungus ability to form functional penetration structures was shown, providing new potential directions for plant disease control strategies.

中文翻译：

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Can1（MCC）和Esosome子域所占据的膜区室在坏死性真菌芸苔Alternaria braciicicola的植物致病性中的作用。

背景技术MCC /同质体是已提出参与质膜功能的膜微区，特别是通过调节脂质的体内平衡，促进特定蛋白质的募集并充当膜储库的提供者。结果在这里我们表明，当发芽的孢子暴露于抗菌防御化合物，渗透压和水分胁迫下时，坏死性真菌A. brasicicola中的几个潜在的MCC /酶体蛋白编码基因被过表达，这是真菌在植物定植过程中遇到的主要制约因素。与参考菌株相比，缺乏关键MCC /同工酶体成分的突变体对植物抗毒素和所施加的胁迫条件的敏感性均未增强。除了Δabpil1a-abpil1b菌株对2 M山梨醇有轻微的超敏性外。根据所考虑的突变体，我们表明与野生型相比，叶片和长角果的定殖过程受到了损害，并假定这些侵略性缺陷可能是由于降低了ress的形成率所致。结论这是关于MCC /酶体在植物病原性真菌致病过程中的作用的首次研究。显示了这些膜结构域与真菌形成功能性渗透结构的能力之间的联系，为植物病害控制策略提供了新的潜在方向。并假设这些侵略性缺陷可能是由于降低了前庭的形成速度所致。结论这是关于MCC /酶体在植物病原性真菌致病过程中的作用的首次研究。显示了这些膜结构域与真菌形成功能性渗透结构的能力之间的联系，为植物病害控制策略提供了新的潜在方向。并假设这些侵略性缺陷可能是由于降低了前庭的形成速度所致。结论这是关于MCC /酶体在植物病原性真菌致病过程中的作用的首次研究。显示了这些膜结构域与真菌形成功能性渗透结构的能力之间的联系，为植物病害控制策略提供了新的潜在方向。