Engineered BRET-Based Biologic Light Sources Enable Spatiotemporal Control over Diverse Optogenetic Systems.,ACS Synthetic Biology

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Engineered BRET-Based Biologic Light Sources Enable Spatiotemporal Control over Diverse Optogenetic Systems.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2019-12-17 , DOI: 10.1021/acssynbio.9b00277
Kshitij Parag-Sharma , Colin P O'Banion _{1,

2} , Erin C Henry ₃ , Adele M Musicant , John L Cleveland ₄ , David S Lawrence _{1,

5} , Antonio L Amelio

Affiliation

Light-inducible optogenetic systems offer precise spatiotemporal control over a myriad of biologic processes. Unfortunately, current systems are inherently limited by their dependence on external light sources for their activation. Further, the utility of laser/LED-based illumination strategies are often constrained by the need for invasive surgical procedures to deliver such devices and local heat production, photobleaching and phototoxicity that compromises cell and tissue viability. To overcome these limitations, we developed a novel BRET-activated optogenetics (BEACON) system that employs biologic light to control optogenetic tools. BEACON is driven by self-illuminating bioluminescent-fluorescent proteins that generate "spectrally tuned" biologic light via bioluminescence resonance energy transfer (BRET). Notably, BEACON robustly activates a variety of commonly used optogenetic systems in a spatially restricted fashion, and at physiologically relevant time scales, to levels that are achieved by conventional laser/LED light sources.

中文翻译：

基于 BRET 的工程生物光源可实现对不同光遗传学系统的时空控制。

光诱导光遗传学系统提供对无数生物过程的精确时空控制。不幸的是，当前的系统本质上受到其激活依赖于外部光源的限制。此外，基于激光/LED的照明策略的实用性通常受到需要侵入性外科手术来提供此类设备以及损害细胞和组织活力的局部产热、光漂白和光毒性的限制。为了克服这些限制，我们开发了一种新型 BRET 激活光遗传学 (BEACON) 系统，该系统利用生物光来控制光遗传学工具。BEACON 由自发光生物发光荧光蛋白驱动，通过生物发光共振能量转移 (BRET) 产生“光谱调谐”生物光。值得注意的是，BEACON 以空间受限的方式和生理相关的时间尺度，稳健地激活各种常用的光遗传学系统，达到传统激光/LED 光源达到的水平。

更新日期：2019-12-18

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