Prosthetic joint infection (PJI) is one of the most devastating failures in total joint replacement (TJR). Infections are becoming difficult to treat due to the emergence of multi-drug resistant bacteria. These bacteria produce biofilm on the implant surface, rendering many antibiotics ineffective by compromising drug diffusion and penetration into the infected area. With the introduction of new antibiotics there is a need to create benchmark data from the traditional antibiotic loaded bone cements. Vancomycin, one of the commonly used antibiotics, shows activity against Methicillin-resistant Staphylococcus aureus (MRSA) and S.epidermidis. In our study, vancomycin added to bone cement was evaluated for elution properties, antimicrobial properties, and mechanical properties of the bone cement. Vancomycin at five different loading masses (0.125, 0.25, 0.5, 1.0 and 2.0 g) was added to 40 g of Simplex™ P cement. Addition of vancomycin affected the mechanical properties and antimicrobial activity with significant differences from controls. Flexural and compression mechanical properties were compromised with added vancomycin. The flexural strength of samples with added vancomycin of 0.5 g and greater were not greater than ISO 5833 minimum requirements. 2.0 g of vancomycin added to bone cement was able to eliminate completely the four bacterial strains tested. 2.0 g of vancomycin also showed the highest mass elution from the cement over a 60-day period. Given the reduced flexural strength in samples with 0.5 g and greater of added vancomycin and the inability of vancomycin in amounts less than 2.0 g to eliminate bacteria, this study did not find an ideal amount of vancomycin added to Simplex™ P that meets both strength and antibacterial requirements.

人工关节感染（PJI）是全关节置换（TJR）中最具破坏性的失败之一。由于多重耐药细菌的出现，感染变得难以治疗。这些细菌在植入物表面产生生物膜，通过损害药物扩散和渗透进入感染区域，使许多抗生素失效。随着新抗生素的引入，有必要从传统的抗生素骨水泥中创建基准数据。万古霉素是一种常用的抗生素，对耐甲氧西林的金黄色葡萄球菌（MRSA）和表皮葡萄球菌具有活性。在我们的研究中，评估了添加到骨水泥中的万古霉素的洗脱性能，抗菌性能和骨水泥的机械​​性能。将五种不同装载质量（0.125、0.25、0.5、1.0和2.0克）的万古霉素添加到40克Simplex™P水泥中。万古霉素的添加影响了机械性能和抗菌活性，与对照组相比有显着差异。加入万古霉素会破坏弯曲和压缩机械性能。添加了0.5 g及更高的万古霉素的样品的抗弯强度不超过ISO 5833的最低要求。向骨水泥中添加2.0 g万古霉素能够完全消除所测试的四种细菌菌株。在60天的时间内，2.0 g万古霉素还显示出最高的水泥洗脱质量。