Acanthopanax senticosus is an important medicinal and edible homologous plant that is widely used in medicines, health products, and functional beverages. Phytochemicals present in plants are partly responsible for various health benefits. Traditional extraction methods use a large number of high-risk organic solvents (acetone, chloroform, etc.) that are potential threats to the environment, human health, and food safety. However, a persistent problem remains in this field with regards to extraction using a simple "green" method. Therefore, a deep eutectic solvent (DES) was used instead of traditional high-risk organic solvents to extract the phytochemicals from Acanthopanax senticosus root. In this study, DES was synthesized from choline chloride and lactic acid, which was used as a substitute for a strong organic solvent to extract saponins, phenylpropionic acid, and terpenoids from Acanthopanax senticosus root. The phytochemicals extracted from Acanthopanax senticosus were analyzed by ultra-high-performance liquid chromatography with quadrupole orbitrap mass spectrometry in positive and negative ionization modes. A total of 91 types of phytochemicals were identified from DES extracts and 17 chemical constituents were newly identified in the root of Acanthopanax senticosus via primary and secondary high-resolution mass spectrometry (MS¹/MS²). Furthermore, DES was demonstrated as a promising functional component of the extraction solvent, and its non-toxic properties endow broad application prospects in the food and health products industries.

刺五加是重要的药用和食用同源植物，广泛用于药物，保健品和功能性饮料。植物中存在的植物化学物质对多种健康有益。传统的提取方法使用大量的高风险有机溶剂（丙酮，氯仿等），这些溶剂可能对环境，人类健康和食品安全构成威胁。然而，在使用简单的“绿色”方法进行提取方面，该领域仍然存在一个持久的问题。因此，使用深共熔溶剂（DES）代替传统的高风险有机溶剂从刺五加中提取植物化学物质。根。在这项研究中，DES是由氯化胆碱和乳酸合成的，它们被用作强有机溶剂的替代品，以从刺五加根中提取皂苷，苯基丙酸和萜类化合物。通过超高效液相色谱-四极杆轨道质谱仪在正电离和负电离模式下对刺五加提取的植物化学成分进行了分析。通过一次和二次高分辨率质谱（MS ¹ / MS ^2）从DES提取物中鉴定出总共91种植物化学物质，并在刺五加的根中新发现了17种化学成分。）。此外，DES被证明是一种有前途的萃取溶剂功能成分，其无毒特性为食品和保健产品行业提供了广阔的应用前景。