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The detection and identification of dengue virus serotypes with quantum dot and AuNP regulated localized surface plasmon resonance
Nanoscale Advances ( IF 4.6 ) Pub Date : 2019/12/13 , DOI: 10.1039/c9na00763f Ankan Dutta Chowdhury 1 , Kenshin Takemura 2 , Indra Memdi Khorish 3 , Fahmida Nasrin 2 , Mya Myat Ngwe Tun 4 , Kouichi Morita 4 , Enoch Y Park 1, 2, 3
Nanoscale Advances ( IF 4.6 ) Pub Date : 2019/12/13 , DOI: 10.1039/c9na00763f Ankan Dutta Chowdhury 1 , Kenshin Takemura 2 , Indra Memdi Khorish 3 , Fahmida Nasrin 2 , Mya Myat Ngwe Tun 4 , Kouichi Morita 4 , Enoch Y Park 1, 2, 3
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The dengue hemorrhagic fever or dengue shock syndrome has become a severe human fatal disease caused by infection with one of the four closely related but serologically distinct dengue viruses (DENVs). All four dengue serotypes are currently co-circulating throughout the subtropics and tropics. Since the fatality rate increases severely when a secondary infection occurs by a virus serotype different from that of the initial infection, serotype identification is equally important as virus detection. In this study, the development and validation of a rapid and quantitative DENV serotype-specific (serotypes 1–4) biosensor are reported by optimizing the stable system between cadmium selenide tellurium sulphide fluorescent quantum dots (CdSeTeS QDs) and gold nanoparticles (AuNPs). Four different nanoprobes are designed using each primer–probe serotype-specific hairpin single-stranded DNA covalently bound at different positions to CdSeTeS QDs, which generates an altered fluorescence signal for each serotype of DENV. In fourplex reactions with free functionalized AuNPs and the four nanoprobes, the standard dilutions of the target virus DNA from 10−15 to 10−10 M were successfully detected. The limit of detection was found to be in the femtomolar range for all four serotypes, where the serotype detection ability was undoubtedly established. To confirm the applicability of this sensing performance in long chained complex RNAs, the sensor was also applied successfully to RNAs extracted from DENV culture fluids for serotype identification as well as quantification, which can lead to a potential diagnostic probe for point-of-care detection.
中文翻译：
用量子点和AuNP调控的局部表面等离子体共振检测和鉴定登革热病毒血清型
登革出血热或登革休克综合征已成为一种严重的人类致命疾病，由感染四种密切相关但血清学不同的登革病毒 (DENV) 之一引起。所有四种登革热血清型目前在亚热带和热带地区共同传播。由于与初次感染不同的病毒血清型发生二次感染时，致死率会急剧增加，因此血清型鉴定与病毒检测同样重要。在本研究中，通过优化硒化镉碲硫化物荧光量子点 (CdSeTeS QDs) 和金纳米粒子 (AuNPs) 之间的稳定系统，报告了一种快速和定量的 DENV 血清型特异性（血清型 1-4）生物传感器的开发和验证。设计了四种不同的纳米探针，使用每种引物-探针血清型特异性发夹单链 DNA 在不同位置与 CdSeTeS QD 共价结合，从而为每种 DENV 血清型产生改变的荧光信号。在使用自由功能化 AuNP 和四个纳米探针的四重反应中，目标病毒 DNA 的标准稀释度为 10-15到 10 -10 M 被成功检测到。发现所有四种血清型的检测限都在飞摩尔范围内，这无疑确立了血清型检测能力。为了确认这种传感性能在长链复杂 RNA 中的适用性，该传感器还成功地应用于从 DENV 培养液中提取的 RNA，用于血清型鉴定和量化，这可能会导致潜在的诊断探针用于床旁检测.
更新日期：2020-02-19
中文翻译：
用量子点和AuNP调控的局部表面等离子体共振检测和鉴定登革热病毒血清型
登革出血热或登革休克综合征已成为一种严重的人类致命疾病，由感染四种密切相关但血清学不同的登革病毒 (DENV) 之一引起。所有四种登革热血清型目前在亚热带和热带地区共同传播。由于与初次感染不同的病毒血清型发生二次感染时，致死率会急剧增加，因此血清型鉴定与病毒检测同样重要。在本研究中，通过优化硒化镉碲硫化物荧光量子点 (CdSeTeS QDs) 和金纳米粒子 (AuNPs) 之间的稳定系统，报告了一种快速和定量的 DENV 血清型特异性（血清型 1-4）生物传感器的开发和验证。设计了四种不同的纳米探针，使用每种引物-探针血清型特异性发夹单链 DNA 在不同位置与 CdSeTeS QD 共价结合，从而为每种 DENV 血清型产生改变的荧光信号。在使用自由功能化 AuNP 和四个纳米探针的四重反应中，目标病毒 DNA 的标准稀释度为 10-15到 10 -10 M 被成功检测到。发现所有四种血清型的检测限都在飞摩尔范围内，这无疑确立了血清型检测能力。为了确认这种传感性能在长链复杂 RNA 中的适用性，该传感器还成功地应用于从 DENV 培养液中提取的 RNA，用于血清型鉴定和量化，这可能会导致潜在的诊断探针用于床旁检测.
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