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A competitive electrochemical aptamer-based method for aflatoxin B1 detection with signal-off response
Analytical Methods ( IF 2.7 ) Pub Date : 2019/12/13 , DOI: 10.1039/c9ay02276g Chao Wang 1, 2, 3, 4, 5 , Yapiao Li 1, 2, 3, 4, 5 , Qiang Zhao 1, 2, 3, 4, 5
Analytical Methods ( IF 2.7 ) Pub Date : 2019/12/13 , DOI: 10.1039/c9ay02276g Chao Wang 1, 2, 3, 4, 5 , Yapiao Li 1, 2, 3, 4, 5 , Qiang Zhao 1, 2, 3, 4, 5
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The authors described a competitive electrochemical aptamer-based method for detection of aflatoxin B1 (AFB1), one of the most toxic mycotoxins. In brief, a complementary DNA (cDNA) strand of an anti-AFB1 aptamer was immobilized on the surface of a gold electrode, and subsequently incubated with sample solution containing a methylene blue (MB)-labeled aptamer. In the absence of AFB1, the MB-labeled aptamer hybridized with the cDNA on the gold electrode, which brought MB close to the gold electrode surface, causing high current. When AFB1 existed in the sample solution, the MB-labeled aptamer bound with AFB1 instead of cDNA, and the amount of MB-labeled aptamer on the gold electrode decreased, so the current signal reduced. Under optimized experimental conditions, the sensor exhibited a detection limit of 2 nM AFB1 and a dynamic detection range of 2 nM to 500 nM. A simple incubation in hot water (85 °C) allowed quick regeneration of the electrochemical sensor. Successful detection of AFB1 spiked in white grape wine demonstrated the application potential of this electrochemical sensor in real-sample analysis.
中文翻译：
一种基于竞争性电化学适体的黄曲霉毒素B1检测方法，具有信号关闭响应
作者介绍了一种基于竞争性电化学适体的方法，用于检测毒性最高的霉菌毒素之一黄曲霉毒素B1（AFB1）。简而言之，将抗AFB1适体的互补DNA（cDNA）链固定在金电极的表面，然后与含有亚甲基蓝（MB）标记的适体的样品溶液一起孵育。在缺少AFB1的情况下，MB标记的适体与金电极上的cDNA杂交，使MB靠近金电极表面，从而产生高电流。当样品溶液中存在AFB1时，MB标记的适体与AFB1结合而不是cDNA结合，金电极上MB标记的适体的数量减少，因此电流信号减少。在优化的实验条件下，该传感器的检测极限为2 nM AFB1，动态检测范围为2 nM至500 nM。在热水（85°C）中进行简单的孵育可以使电化学传感器快速再生。成功检测到白葡萄酒中加标的AFB1，证明了该电化学传感器在实际样品分析中的应用潜力。
更新日期：2020-02-13
中文翻译：
一种基于竞争性电化学适体的黄曲霉毒素B1检测方法，具有信号关闭响应
作者介绍了一种基于竞争性电化学适体的方法，用于检测毒性最高的霉菌毒素之一黄曲霉毒素B1（AFB1）。简而言之，将抗AFB1适体的互补DNA（cDNA）链固定在金电极的表面，然后与含有亚甲基蓝（MB）标记的适体的样品溶液一起孵育。在缺少AFB1的情况下，MB标记的适体与金电极上的cDNA杂交，使MB靠近金电极表面，从而产生高电流。当样品溶液中存在AFB1时，MB标记的适体与AFB1结合而不是cDNA结合，金电极上MB标记的适体的数量减少，因此电流信号减少。在优化的实验条件下，该传感器的检测极限为2 nM AFB1，动态检测范围为2 nM至500 nM。在热水（85°C）中进行简单的孵育可以使电化学传感器快速再生。成功检测到白葡萄酒中加标的AFB1，证明了该电化学传感器在实际样品分析中的应用潜力。
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