Supramolecular Tandem Assay for Pyridoxal-5'-phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein.,ChemistryOpen

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Supramolecular Tandem Assay for Pyridoxal-5'-phosphate by the Reporter Pair of Guanidinocalix[5]Arene and Fluorescein.
ChemistryOpen ( IF 2.5 ) Pub Date : 2019-12-13 , DOI: 10.1002/open.201900316
Yu-Xin Yue ₁ , Yong Kong ₂ , Fan Yang ₂ , Zhe Zheng ₁ , Xin-Yue Hu ₁ , Dong-Sheng Guo ₁

Affiliation

Guanidinocalix[5]arene and fluorescein reporter pair has been chosen to set up a supramolecular tandem assay principle based on the differential recognition of pyridoxal‐5′‐phosphate (the substrate of alkaline phosphatase, ALP), pyridoxal (the product of ALP) and phosphate (the product of ALP). This supramolecular tandem assay system offers an opportunity to monitor the activity of ALP in a label‐free, continuous, and real‐time manner. More importantly, a calibration curve can be given for selective and quantitative detection of pyridoxal‐5′‐phosphate (biomarker for several diseases).

中文翻译：

通过胍基杯[5]芳烃和荧光素报告基因对对 5'-磷酸吡哆醛进行超分子串联测定。

选择胍基杯[5]芳烃和荧光素报告对建立基于吡哆醛-5'-磷酸（碱性磷酸酶的底物，ALP）、吡哆醛（ALP的产物）和吡哆醛（ALP的产物）的差异识别的超分子串联检测原理。磷酸盐（ALP 的产物）。这种超分子串联测定系统提供了以无标记、连续和实时的方式监测 ALP 活性的机会。更重要的是，可以给出校准曲线，用于选择性和定量检测吡哆醛-5'-磷酸（多种疾病的生物标志物）。

更新日期：2019-12-13

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