Our group has reported the imprint formation of biological material on polycarbonate nuclear track detectors by UV-C exposure, which is used as an approach to simultaneously visualize cell imprints and nuclear tracks coming from the boron neutron capture reaction. Considering that the cell nucleus has a higher UV-C absorption than the cytoplasm and that hematoxylin preferentially stains the nucleus, we proposed to enhance the contrast between these two main cell structures by hematoxylin staining before UV-C sensitization. In this study, several experiments were performed in order to optimize UV-C exposure parameters and chemical etching conditions for cell imprint formation using the SK-BR-3 breast cancer cell line. The proposed method improves significantly the resolution of the cell imprints. It allows clear differentiation of the nucleus from the rest of the cell, together with nuclear tracks pits. Moreover, it reduces considerably the UV-C exposure time, an important experimental issue. The proposed methodology can be applied to study the boron distribution independently from the chosen cell line and/or boron compounds.

中文翻译：

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中子放射自显影结合 UV-C 敏化：走向硼的细胞内定位

我们小组报告了通过 UV-C 暴露在聚碳酸酯核轨道探测器上形成生物材料的印记，这是一种同时可视化来自硼中子捕获反应的细胞印记和核轨道的方法。考虑到细胞核比细胞质具有更高的 UV-C 吸收，并且苏木精优先染色细胞核，我们建议在 UV-C 敏化之前通过苏木精染色来增强这两种主要细胞结构之间的对比度。在这项研究中，为了优化使用 SK-BR-3 乳腺癌细胞系形成细胞印记的 UV-C 暴露参数和化学蚀刻条件，进行了几项实验。所提出的方法显着提高了细胞印迹的分辨率。它可以清楚地区分细胞核与细胞的其余部分，以及核轨道凹坑。此外，它大大减少了 UV-C 曝光时间，这是一个重要的实验问题。所提出的方法可用于独立于所选细胞系和/或硼化合物研究硼分布。