The roundworms of Trichinella genus are worldwide distributed and their prevalence in nature is high. Trichinella genus parasites are the causative agents of foodborne zoonosis trichinellosis. The main prevention and control of the infection are meat inspection by the magnetic stirrer method for the detection of Trichinella larvae in muscle samples. The treatment can be effective if the parasite is discovered early in the intestinal phase. Once the Trichinella larva has reached the muscle tissue, the parasite remains therein and there is no treatment for this life cycle stage. The Trichinella species is dioecious with separate male and female individuals. The developed staining technique that uses confocal laser scanning microscopy (CLSM) displays sufficient results for Trichinella larvae examination and this protocol is applicable to study the internal and external structures and for the sex determination of T. britovi and T. spiralis larvae samples. In the present study, a luminescent derivative was synthesized and used for staining of T. spiralis and T. britovi larvae samples for the examination by CLSM. Various fixatives, such as AFA, 70% ethanol, and Bouin's and Carnoy's solutions were tested for sample preparation. The synthesized luminescent compound demonstrates best visualization results for samples fixed in Bouin's fixative.

中文翻译：

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用激光共聚焦扫描显微镜对旋毛虫幼虫寄生虫进行性别鉴定的简单快速染色技术

蛔虫旋毛虫该属分布于世界各地，在自然界中流行率很高。旋毛虫寄生虫属是食源性人畜共患病旋毛虫病的病原体。感染的主要预防和控制是通过磁力搅拌器方法检测肉类旋毛虫肌肉样本中的幼虫。如果在肠道早期发现寄生虫，治疗会很有效。一旦旋毛虫幼虫已到达肌肉组织，寄生虫仍留在其中，并且没有针对此生命周期阶段的治疗。这旋毛虫物种是雌雄异株的，有单独的雄性和雌性个体。使用共聚焦激光扫描显微镜 (CLSM) 开发的染色技术显示了足够的结果旋毛虫幼虫检查和本协议适用于研究内部和外部结构和确定性别T. britovi和T.spiralis幼虫样品。在本研究中，合成了一种发光衍生物并用于染色T.spiralis和T. britoviCLSM 检查的幼虫样本。测试了各种固定剂，例如 AFA、70% 乙醇以及 Bouin 和 Carnoy 溶液以进行样品制备。合成的发光化合物对在 Bouin 固定剂中固定的样品显示出最佳的可视化效果。