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Biological activities of the essential oil from the Moro orange peel (Citrus sinensis (L.) Osbeck)
Flavour and Fragrance Journal ( IF 2.1 ) Pub Date : 2019-12-11 , DOI: 10.1002/ffj.3561 Maísa Lamounier Magalhães 1 , Marisa Ionta 2 , Guilherme Álvaro Ferreira 2 , Marina Leopoldina Lamounier Campidelli 1 , David Lee Nelson 3 , Vanuzia Rodrigues Fernandes Ferreira 4 , Danubia Aparecida de Carvalho Selva Rezende 4 , Maria das Graças Cardoso 4
Flavour and Fragrance Journal ( IF 2.1 ) Pub Date : 2019-12-11 , DOI: 10.1002/ffj.3561 Maísa Lamounier Magalhães 1 , Marisa Ionta 2 , Guilherme Álvaro Ferreira 2 , Marina Leopoldina Lamounier Campidelli 1 , David Lee Nelson 3 , Vanuzia Rodrigues Fernandes Ferreira 4 , Danubia Aparecida de Carvalho Selva Rezende 4 , Maria das Graças Cardoso 4
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Citrus essential oils have become the focus of several researches, because they have broad biological activity, due to their chemical composition. However, there are few studies covering Moro orange essential oil. Thus, the aim of this study was to evaluate the effects of fresh Moro orange peel essential oil on antifungal, antioxidant, cytotoxic, and genotoxic activities in normal and tumor cells. The essential oil was extracted by hydrodistillation and characterized by GC/MS and GC/FID. Cytotoxicity was evaluated by MTS assay in normal cells (CCD‐1059Sk) and tumor cells (CCD‐1059Sk), and the genotoxicity was determined in normal cells by the comet assay. Antifungal activity was evaluated by the disk diffusion test against Aspergillus carbonarius and Aspergillus flavus filamentous fungi. The antioxidant activity was determined in DPPH, ABTS, and β‐carotene assay. The essential oil was mainly composed of monoterpenes such as limonene (95.12%), α‐pinene (0.35%), sabinene (0.54%), and myrcene (1.07%). The essential oil did not cause cell death after 48 hours of treatment in normal cells, but the cell viability of tumor cells decreased by 50% in the presence of the essential oil at a concentration of 272.6 μg/mL. Although no antioxidant effect was observed, the growth of all evaluated fungi was inhibited with a minimum inhibitory concentration (MIC) of 125 μL/mL. The essential oil from the Moro orange is promising for cancer treatment, and it can be used as a natural preservative in food systems.
中文翻译：
摩洛橙皮（Citrus sinensis（L.）Osbeck）精油的生物活性
柑橘精油由于其化学成分而具有广泛的生物活性，因此已成为数项研究的重点。但是，很少有研究涵盖摩洛橙精油。因此，本研究的目的是评估新鲜的摩洛橙皮精油对正常细胞和肿瘤细胞的抗真菌，抗氧化剂，细胞毒性和基因毒性的作用。通过加氢蒸馏提取精油，并通过GC / MS和GC / FID进行表征。通过MTS分析评估正常细胞（CCD-1059Sk）和肿瘤细胞（CCD-1059Sk）的细胞毒性，并通过彗星分析确定正常细胞的遗传毒性。通过圆盘扩散试验评估了对碳曲霉和黄曲霉的抗真菌活性。丝状真菌。抗氧化剂活性通过DPPH，ABTS和β-胡萝卜素测定来确定。精油主要由单萜烯组成，例如柠檬烯（95.12％），α-pine烯（0.35％），sa烯（0.54％）和月桂烯（1.07％）。在正常细胞中处理48小时后，精油没有引起细胞死亡，但是在浓度为272.6μg/ mL的精油存在下，肿瘤细胞的细胞活力降低了50％。尽管未观察到抗氧化作用，但所有评估真菌的生长均以125μL/ mL的最小抑菌浓度（MIC）受到抑制。摩洛橙的精油有望用于癌症治疗，可在食品系统中用作天然防腐剂。
更新日期：2019-12-11
中文翻译：
摩洛橙皮（Citrus sinensis（L.）Osbeck）精油的生物活性
柑橘精油由于其化学成分而具有广泛的生物活性，因此已成为数项研究的重点。但是，很少有研究涵盖摩洛橙精油。因此，本研究的目的是评估新鲜的摩洛橙皮精油对正常细胞和肿瘤细胞的抗真菌，抗氧化剂，细胞毒性和基因毒性的作用。通过加氢蒸馏提取精油，并通过GC / MS和GC / FID进行表征。通过MTS分析评估正常细胞（CCD-1059Sk）和肿瘤细胞（CCD-1059Sk）的细胞毒性，并通过彗星分析确定正常细胞的遗传毒性。通过圆盘扩散试验评估了对碳曲霉和黄曲霉的抗真菌活性。丝状真菌。抗氧化剂活性通过DPPH，ABTS和β-胡萝卜素测定来确定。精油主要由单萜烯组成，例如柠檬烯（95.12％），α-pine烯（0.35％），sa烯（0.54％）和月桂烯（1.07％）。在正常细胞中处理48小时后，精油没有引起细胞死亡，但是在浓度为272.6μg/ mL的精油存在下，肿瘤细胞的细胞活力降低了50％。尽管未观察到抗氧化作用，但所有评估真菌的生长均以125μL/ mL的最小抑菌浓度（MIC）受到抑制。摩洛橙的精油有望用于癌症治疗，可在食品系统中用作天然防腐剂。
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