Hemoglobin A1c (HbA1c) is a reliable marker of insulin resistance in normal glucose tolerance patients; however, several physiological, environmental, and genetic factors may affect HbA1c and cause false results. Therefore, it is essential to use new biomarkers due to increasing diabetes predictive value. Recently, it has been indicated that microRNAs (miRNAs) are involved in the pathophysiology of diabetes, particularly, in insulin resistance pathways. Therefore, miRNAs have the potential to be introduced as new glycemic control biomarkers. The aim of this study was to investigate the association between plasma level of miRNA-135a and HbA1c in patients with prediabetes and type 2 diabetes. In this case-control study, 120 samples were enrolled (healthy individuals, people with type 2 diabetes, and prediabetes) and HbA1c and miR-135a expression level in their plasma samples were evaluated. Multinomial logistic regression and ROC curve analysis were conducted to assess the diagnostic accuracy of plasma miR-135a in T2D , prediabetes, and healthy control groups. Data analysis indicated that miR-135a was significantly elevated in both diabetes/prediabetes samples. Then, subjects were reclassified based on the calculated cutoff value of miRNA. Logistic Regression analysis showed that an increased level of miRNA positively correlated with HbA1c level in prediabetes (Adjusted OR = 1.14, p value = 0.033) and diabetic status (Adjusted OR = 1.27, p value = 0.024 ). miR-135 may provide an assistant marker for HbA1c to detect type 2 diabetes.

中文翻译：

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循环中的miR-135可作为2型糖尿病中HbA1c的新型共生标志物。

血红蛋白A1c（HbA1c）是正常葡萄糖耐量患者胰岛素抵抗的可靠标志物；但是，一些生理，环境和遗传因素可能会影响HbA1c并导致错误的结果。因此，由于增加了糖尿病的预测价值，因此必须使用新的生物标志物。最近，已经表明，微小RNA（miRNA）参与糖尿病的病理生理，特别是胰岛素抵抗途径。因此，miRNAs有可能被引入为新的血糖控制生物标志物。这项研究的目的是调查前驱糖尿病和2型糖尿病患者的miRNA-135a和HbA1c血浆水平之间的关系。在该病例对照研究中，纳入了120个样本（健康个体，2型糖尿病患者，和糖尿病前期）以及血浆样品中HbA1c和miR-135a的表达水平。进行了多项logistic回归和ROC曲线分析，以评估血浆miR-135a在T2D，糖尿病前期和健康对照组中的诊断准确性。数据分析表明，在糖尿病/前驱糖尿病样品中，miR-135a均显着升高。然后，根据计算出的miRNA临界值对受试者进行重新分类。Logistic回归分析显示，在前驱糖尿病中，miRNA的水平升高与HbA1c水平呈正相关（调整后的OR = 1.14，数据分析表明，在糖尿病/前驱糖尿病样品中，miR-135a均显着升高。然后，根据计算出的miRNA临界值对受试者进行重新分类。Logistic回归分析显示，在前驱糖尿病中，miRNA的水平升高与HbA1c水平呈正相关（调整后的OR = 1.14，数据分析表明，在糖尿病/前驱糖尿病样品中，miR-135a均显着升高。然后，根据计算出的miRNA临界值对受试者进行重新分类。Logistic回归分析显示，在前驱糖尿病中，miRNA的水平升高与HbA1c水平呈正相关（校正后的OR = 1.14，p值= 0.033）和糖尿病状态（调整OR = 1.27，p值= 0.024）。miR-135可为HbA1c提供辅助标记，以检测2型糖尿病。