Proteolytic enzymes and their homologues have been classified into clans by comparing the tertiary structures of the peptidase domains, into families by comparing the protein sequences of the peptidase domains, and into protein-species by comparing various attributes including domain architecture, substrate preference, inhibitor interactions, subcellular location, and phylogeny. The results are compared with the earlier classification (Rawlings and Barrett, 1993 [1]). The numbers of sequences, protein-species, families, clans and even catalytic type have substantially increased during the intervening 26 years. The alternative classifications by catalytic type and/or activity are shown not to reflect evolutionary relationships.

中文翻译：

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二十五年蛋白水解酶的命名和分类。

通过比较肽酶结构域的三级结构，将蛋白水解酶及其同源物分类为氏族，通过比较肽酶结构域的蛋白序列将其划分为家族，通过比较包括结构域结构，底物偏好，抑制剂相互作用在内的各种属性，将其分为蛋白质种类，亚细胞定位和系统发育。将结果与早期分类进行比较（Rawlings和Barrett，1993 [1]）。在过去的26年中，序列，蛋白质种类，家族，氏族甚至催化类型的数量已大大增加。显示了通过催化类型和/或活性的替代分类不反映进化关系。