Bisphenol A (BPA), a known potential endocrine disrupting compound (EDC) is expected to be present in low quantities in canned food due to its migration from the inner surface coating of cans made of epoxy resins. A selective and confirmatory analytical method, based on microwave assisted extraction (MAE), molecularly imprinted solid phase extraction (MISPE) using a polymer prepared by a non-covalent molecular imprinting technique and liquid chromatography coupled with electrospray ionization mass spectrometry (LC-ESI/MS) was developed for the determination of BPA in canned pineapple, tuna and mushrooms. First, the effect of the loading medium of hydro- organic solutions on the binding of BPA and its deuterated analogue on the MISPE sorbent was investigated. Subsequently, the effects of the experimental conditions of the microwave assisted extraction (solvent, sample mass/solvent volume, time and temperature) on the obtained recovery of BPA from canned food were assessed and the parameters were optimized to provide maximum recovery and selectivity. It was demonstrated that the combination of MAE with MISPE permits the use of a selective extraction solvent (methanol/water, 4/6, v/v), simplifying the sample preparation steps and enhancing sample clean-up of complex food matrices. The method was validated in different food matrices, using BPA-d16 as internal standard and quantitative relative recoveries were determined. The precision (RSD %) of the method ranged from 7% to 10% and the limit of detection was at low ng/g level for all food matrices. The determined concentration of BPA in commercial canned samples ranged between 7.3 – 42.3 ng/g.

双酚A（BPA）是一种已知的潜在内分泌干扰化合物（EDC），由于其从环氧树脂制成的罐的内表面涂层中迁移出来，因此预计在罐装食品中将以少量存在。基于微波辅助萃取（MAE），分子印迹固相萃取（MISPE）的选择性和确证分析方法，该方法使用通过非共价分子印迹技术制备的聚合物和液相色谱结合电喷雾电离质谱法（LC-ESI / MS）用于测定菠萝罐头，金枪鱼和蘑菇中的BPA。首先，研究了水性有机物上样介质对BPA及其氘代类似物在MISPE吸附剂上结合的影响。随后，评估了微波辅助萃取的实验条件（溶剂，样品质量/溶剂体积，时间和温度）对从罐头食品中获得的BPA回收率的影响，并优化了参数以提供最大的回收率和选择性。事实证明，MAE与MISPE的组合允许使用选择性萃取溶剂（甲醇/水，4/6，v / v），简化了样品制备步骤，并增强了复杂食品基质的样品净化能力。使用BPA-d16作为内标，该方法已在不同食品基质中得到验证，并确定了定量相对回收率。该方法的精密度（RSD％）为7％至10％，所有食品基质的检出限均为ng / g低。