Point-of-Use Detection of Environmental Fluoride via a Cell-Free Riboswitch-Based Biosensor.,ACS Synthetic Biology

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Point-of-Use Detection of Environmental Fluoride via a Cell-Free Riboswitch-Based Biosensor.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2019-12-20 , DOI: 10.1021/acssynbio.9b00347
Walter Thavarajah _{1,

2,

3} , Adam D Silverman _{1,

2,

3} , Matthew S Verosloff _{2,

3,

4} , Nancy Kelley-Loughnane ₅ , Michael C Jewett _{1,

2} , Julius B Lucks _{1,

2,

3}

Affiliation

Advances in biosensor engineering have enabled the design of programmable molecular systems to detect a range of pathogens, nucleic acids, and chemicals. Here, we engineer and field-test a biosensor for fluoride, a major groundwater contaminant of global concern. The sensor consists of a cell-free system containing a DNA template that encodes a fluoride-responsive riboswitch regulating genes that produce a fluorescent or colorimetric output. Individual reactions can be lyophilized for long-term storage and detect fluoride at levels above 2 ppm, the Environmental Protection Agency's most stringent regulatory standard, in both laboratory and field conditions. Through onsite detection of fluoride in a real-world water source, this work provides a critical proof-of-principle for the future engineering of riboswitches and other biosensors to address challenges for global health and the environment.

中文翻译：

通过基于无细胞核糖开关的生物传感器对环境氟化物进行使用点检测。

生物传感器工程的进步使得可编程分子系统的设计能够检测一系列病原体、核酸和化学物质。在这里，我们设计并现场测试了氟化物生物传感器，氟化物是全球关注的主要地下水污染物。该传感器由含有 DNA 模板的无细胞系统组成，该模板编码氟化物响应核糖开关调节基因，从而产生荧光或比色输出。单个反应可以冻干以便长期储存，并在实验室和现场条件下检测浓度高于 2 ppm 的氟化物，这是环境保护局最严格的监管标准。通过现场检测现实世界水源中的氟化物，这项工作为核糖开关和其他生物传感器的未来工程提供了关键的原理验证，以应对全球健康和环境的挑战。

更新日期：2019-12-21

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