Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Time-Resolved Monitoring of Intracellular Processes with a Cyclical On-Off Photoswitchable Nanoprobe.
ACS Sensors ( IF 8.2 ) Pub Date : 2019-12-23 , DOI: 10.1021/acssensors.9b01182 Xu Jie 1 , Haimei Yang 1 , Yuchen Su 1 , Zhining Xia 1 , Weili Wei 1
ACS Sensors ( IF 8.2 ) Pub Date : 2019-12-23 , DOI: 10.1021/acssensors.9b01182 Xu Jie 1 , Haimei Yang 1 , Yuchen Su 1 , Zhining Xia 1 , Weili Wei 1
Affiliation
|
Fluorescent microscopic imaging with the help of small-molecule probes (chemoprobes) is one of the most feasible approaches for noninvasive sensing of intracellular molecules. However, the "always on" property of current chemoprobes failed to achieve time-resolved monitoring. Here, we report the development of a supramolecular nanoassembling strategy to integrate multiple functions on one nanoscale probe (nanoprobe) with a cyclical on-off switchable sensing ability. The reversal of the nanoprobe can be rapidly achieved by converting the single-wavelength near-infrared (NIR) laser to two-way emissions by a lanthanum nanoparticle core that is sensitive to the light power density. Through regulating the NIR power density, the azobenzene derivative, which was doped in the surface of the lipid bilayer of the nanoprobe, can act as an "impeller" and "brake" for bio-benign activation and deactivation, respectively, of the nanoprobe in biological applications. A reduced nicotinamide adenine dinucleotide nanoprobe was constructed as the model to demonstrate precise and time-resolved monitoring of intracellular processes including cancerous glycolysis and ligand-induced enzymatic processes. We envision that this cyclical on-off switchable nanoprobe strategy will hold great promise for endowing universal chemoprobes with high precision and temporal resolution.
中文翻译：
时间分辨监测细胞内过程与循环开关光开关纳米探针。
借助小分子探针（化学探针）的荧光显微成像是用于细胞内分子无创检测的最可行方法之一。但是，当前化学探针的“ always on”（始终在线）属性无法实现时间分辨的监视。在这里，我们报告超分子纳米组装策略的发展，以整合具有循环开-关可切换传感能力的一个纳米级探针（纳米探针）上的多种功能。通过对光功率密度敏感的镧纳米粒子核将单波长近红外（NIR）激光转换为双向发射，可以快速实现纳米探针的反转。通过调节NIR功率密度，掺杂在纳米探针脂质双层表面上的偶氮苯衍生物可以充当“ 构建还原的烟酰胺腺嘌呤二核苷酸纳米探针作为模型，以展示精确且时间分辨的细胞内过程监测，包括癌性糖酵解和配体诱导的酶促过程。我们预想，这种周期性的开-关可切换纳米探针策略将为以高精度和高时间分辨率赋予通用化学探针提供广阔的前景。构建还原的烟酰胺腺嘌呤二核苷酸纳米探针作为模型，以展示精确且时间分辨的细胞内过程监测，包括癌性糖酵解和配体诱导的酶促过程。我们预想，这种周期性的开-关可切换纳米探针策略将为以高精度和高时间分辨率赋予通用化学探针提供广阔的前景。
更新日期：2019-12-25
中文翻译：
时间分辨监测细胞内过程与循环开关光开关纳米探针。
借助小分子探针（化学探针）的荧光显微成像是用于细胞内分子无创检测的最可行方法之一。但是，当前化学探针的“ always on”（始终在线）属性无法实现时间分辨的监视。在这里，我们报告超分子纳米组装策略的发展，以整合具有循环开-关可切换传感能力的一个纳米级探针（纳米探针）上的多种功能。通过对光功率密度敏感的镧纳米粒子核将单波长近红外（NIR）激光转换为双向发射，可以快速实现纳米探针的反转。通过调节NIR功率密度，掺杂在纳米探针脂质双层表面上的偶氮苯衍生物可以充当“ 构建还原的烟酰胺腺嘌呤二核苷酸纳米探针作为模型，以展示精确且时间分辨的细胞内过程监测，包括癌性糖酵解和配体诱导的酶促过程。我们预想，这种周期性的开-关可切换纳米探针策略将为以高精度和高时间分辨率赋予通用化学探针提供广阔的前景。构建还原的烟酰胺腺嘌呤二核苷酸纳米探针作为模型，以展示精确且时间分辨的细胞内过程监测，包括癌性糖酵解和配体诱导的酶促过程。我们预想，这种周期性的开-关可切换纳米探针策略将为以高精度和高时间分辨率赋予通用化学探针提供广阔的前景。
京公网安备 11010802027423号