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Fast and Reproducible ELISA Laser Platform for Ultrasensitive Protein Quantification.
ACS Sensors ( IF 8.2 ) Pub Date : 2019-12-23 , DOI: 10.1021/acssensors.9b01795 Xiaotian Tan 1 , Qiushu Chen 1 , Hongbo Zhu 1 , Song Zhu 1, 2 , Yuan Gong 3 , Xiaoqin Wu 1 , Yu-Cheng Chen 1, 4 , Xuzhou Li 1 , Maxwell Wei-Hao Li 1 , Wenyi Liu 1 , Xudong Fan 1
ACS Sensors ( IF 8.2 ) Pub Date : 2019-12-23 , DOI: 10.1021/acssensors.9b01795 Xiaotian Tan 1 , Qiushu Chen 1 , Hongbo Zhu 1 , Song Zhu 1, 2 , Yuan Gong 3 , Xiaoqin Wu 1 , Yu-Cheng Chen 1, 4 , Xuzhou Li 1 , Maxwell Wei-Hao Li 1 , Wenyi Liu 1 , Xudong Fan 1
Affiliation
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Optofluidic lasers are currently of high interest for sensitive intracavity biochemical analysis. In comparison with conventional methods such as fluorescence and colorimetric detection, optofluidic lasers provide a method for amplifying small concentration differences in the gain medium, thus achieving high sensitivity. Here, we report the development of an on-chip ELISA (enzyme-linked immunosorbent assay) laser platform that is able to complete an assay in a short amount of time with small sample/reagent volumes, large dynamic range, and high sensitivity. The arrayed microscale reaction wells in the ELISA lasers can be microfabricated directly on dielectric mirrors, thus significantly improving the quality of the reaction wells and detection reproducibility. The details of the fabrication and characterization of those reaction wells on the mirror are described and the ELISA laser assay protocols are developed. Finally, we applied the ELISA laser to detecting IL-6, showing that a detection limit of about 0.1 pg/mL can be achieved in 1.5 h with 15 μL of sample/reagents per well. This work pushes the ELISA laser a step closer to solving problems in real-world biochemical analysis.
中文翻译：
快速且可重现的ELISA激光平台，用于超灵敏蛋白质定量。
当前，对于敏感的腔内生化分析，光流体激光器引起了人们的极大兴趣。与诸如荧光和比色检测之类的常规方法相比，光流体激光器提供了一种用于放大增益介质中较小浓度差异的方法，从而实现了高灵敏度。在这里，我们报告了片上ELISA（酶联免疫吸附测定）激光平台的开发，该平台能够在短时间内完成小样品/试剂量，大动态范围和高灵敏度的测定。ELISA激光器中排列的微型反应孔可以直接在介电镜上进行微加工，从而显着提高了反应孔的质量和检测重现性。描述了在镜子上这些反应孔的制造和表征的细节，并开发了ELISA激光测定规程。最后，我们将ELISA激光应用于IL-6的检测，结果表明，每孔15μL样品/试剂可在1.5 h内达到约0.1 pg / mL的检测限。这项工作将ELISA激光推向了解决现实世界中生化分析问题的一步。
更新日期：2019-12-25
中文翻译：
快速且可重现的ELISA激光平台，用于超灵敏蛋白质定量。
当前，对于敏感的腔内生化分析，光流体激光器引起了人们的极大兴趣。与诸如荧光和比色检测之类的常规方法相比，光流体激光器提供了一种用于放大增益介质中较小浓度差异的方法，从而实现了高灵敏度。在这里，我们报告了片上ELISA（酶联免疫吸附测定）激光平台的开发，该平台能够在短时间内完成小样品/试剂量，大动态范围和高灵敏度的测定。ELISA激光器中排列的微型反应孔可以直接在介电镜上进行微加工，从而显着提高了反应孔的质量和检测重现性。描述了在镜子上这些反应孔的制造和表征的细节，并开发了ELISA激光测定规程。最后，我们将ELISA激光应用于IL-6的检测，结果表明，每孔15μL样品/试剂可在1.5 h内达到约0.1 pg / mL的检测限。这项工作将ELISA激光推向了解决现实世界中生化分析问题的一步。
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