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Silver Nanoparticle Conjugated Star PCL-b-AMPs Copolymer as Nanocomposite Exhibits Efficient Antibacterial Properties.
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2019-12-27 , DOI: 10.1021/acs.bioconjchem.9b00739 Jian-Bin Zhen 1 , Peng-Wei Kang 1 , Mu-Han Zhao 1 , Ke-Wu Yang 1
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2019-12-27 , DOI: 10.1021/acs.bioconjchem.9b00739 Jian-Bin Zhen 1 , Peng-Wei Kang 1 , Mu-Han Zhao 1 , Ke-Wu Yang 1
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The traditional antibiotics have specific intracellular targets and disinfect in chemical ways, and the drug-resistance induced by the antibiotics has grown into an emerging threat. It is urgent to call for novel strategies and antibacterial materials to control this situation. Herein, we report a class of silver-decorated nanocomposite AgNPs@PCL-b-AMPs as potent nanoantibiotic, constructed by ring-opening polymerization of the monomers ε-caprolactone, Z-Lys-N-carboxyanhydrides (NCAs), and Phe-NCAs, then decorated with AgNPs, and characterized by SEM, TEM, and DLS. The biological assays revealed that the nanocomposite possessed strong antibacterial efficacy against both Gram-positive and Gram-negative bacteria including clinical isolated bacteria MRSA, VRE, P. aeruginosa, and K. pneumonia, exhibiting a MIC value range in 2-8 μg/mL. Importantly, the S. aureus and P. aeruginosa treated with the nanocomposite did not show drug-resistance even after 21 passages. Also, in vivo anti-infective assays showed that the nanocomposite was able to effectively kill bacteria in the infected viscera of mice. The study of the sterilization mechanism showed that the nanocomposite exhibited a multimodal antimicrobial mechanism, including irreversibly damaging the membrane structure, making the leakage of intracellular ions and subsequently inducing generation of the reactive oxygen species (ROS), ultimately sterilizing the bacteria. The nanocomposite exhibits effective broad-spectrum antibacterial properties and shows low toxicity to the mammalian cells/animal. Overall, the AgNPs@PCL-b-AMPs gained in this work show great potential as a highly promising antibacterial material for biomedical applications including drug-resistant bacterial infection.
中文翻译：
银纳米粒子共轭星PCL-b-AMPs共聚物作为纳米复合材料表现出有效的抗菌性能。
传统的抗生素具有特定的细胞内靶标并以化学方式进行消毒，并且由抗生素引起的耐药性已成为一种新兴的威胁。迫切需要新颖的策略和抗菌材料来控制这种情况。在这里，我们报告一类银修饰的纳米复合AgNPs @ PCL-b-AMPs作为有效的纳米抗生素，它是通过单体ε-己内酯，Z-Lys-N-羧基酐（NCAs）和Phe-NCAs的开环聚合反应构建的，然后用AgNPs装饰，并用SEM，TEM和DLS进行表征。生物学分析表明，该纳米复合材料对革兰氏阳性和革兰氏阴性细菌（包括临床分离的细菌MRSA，VRE，铜绿假单胞菌和肺炎克雷伯氏菌）均具有强大的抗菌作用，MIC值范围为2-8μg/ mL 。重要的，用纳米复合材料处理的金黄色葡萄球菌和铜绿假单胞菌即使经过21代也没有显示出耐药性。同样，体内抗感染试验表明，纳米复合材料能够有效杀死小鼠受感染内脏中的细菌。对杀菌机理的研究表明，纳米复合材料表现出多峰抗菌机制，包括不可逆地破坏膜结构，使细胞内离子泄漏并随后诱导产生活性氧（ROS），最终对细菌进行杀菌。该纳米复合材料表现出有效的广谱抗菌特性，并且对哺乳动物细胞/动物具有低毒性。全面的，
更新日期：2019-12-27
中文翻译：
银纳米粒子共轭星PCL-b-AMPs共聚物作为纳米复合材料表现出有效的抗菌性能。
传统的抗生素具有特定的细胞内靶标并以化学方式进行消毒，并且由抗生素引起的耐药性已成为一种新兴的威胁。迫切需要新颖的策略和抗菌材料来控制这种情况。在这里，我们报告一类银修饰的纳米复合AgNPs @ PCL-b-AMPs作为有效的纳米抗生素，它是通过单体ε-己内酯，Z-Lys-N-羧基酐（NCAs）和Phe-NCAs的开环聚合反应构建的，然后用AgNPs装饰，并用SEM，TEM和DLS进行表征。生物学分析表明，该纳米复合材料对革兰氏阳性和革兰氏阴性细菌（包括临床分离的细菌MRSA，VRE，铜绿假单胞菌和肺炎克雷伯氏菌）均具有强大的抗菌作用，MIC值范围为2-8μg/ mL 。重要的，用纳米复合材料处理的金黄色葡萄球菌和铜绿假单胞菌即使经过21代也没有显示出耐药性。同样，体内抗感染试验表明，纳米复合材料能够有效杀死小鼠受感染内脏中的细菌。对杀菌机理的研究表明，纳米复合材料表现出多峰抗菌机制，包括不可逆地破坏膜结构，使细胞内离子泄漏并随后诱导产生活性氧（ROS），最终对细菌进行杀菌。该纳米复合材料表现出有效的广谱抗菌特性，并且对哺乳动物细胞/动物具有低毒性。全面的，
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