当前位置：
X-MOL 学术
›
Bioconjugate Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Self-Illuminating Photodynamic Therapy with Enhanced Therapeutic Effect by Optimization of the Chemiluminescence Resonance Energy Transfer Step to the Photosensitizer.
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2020-01-08 , DOI: 10.1021/acs.bioconjchem.9b00740 Kun Yang 1 , Chunlai Wang 1 , Xiaohui Wei 1 , Sheng Ding 1 , Changjun Liu 1, 2 , Feng Tian 1 , Fan Li 1
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2020-01-08 , DOI: 10.1021/acs.bioconjchem.9b00740 Kun Yang 1 , Chunlai Wang 1 , Xiaohui Wei 1 , Sheng Ding 1 , Changjun Liu 1, 2 , Feng Tian 1 , Fan Li 1
Affiliation
|
The major obstacles to the wider application of photodynamic therapy (PDT) are drawbacks of the current photosensitizers and the tissue penetration limit of the common outer light source. In the present study, the chemiluminescence (CL) from the luminol-H2O2-horseradish peroxidase reaction was explored as a potential inner light source for the intracellular activation of carbon dots (CDs)-based PDT system. To fully use the light and enhance the overall PDT yield, the nanocarrier of CDs, the light of CL, and the PDT agent chlorin e6 (Ce6) were carefully selected and designed to form an efficient and united system. Bright-yellow-emissive CDs (y-CDs) were synthesized through purposeful regulation of the absorption and emission spectra to enhance the overlapping areas in the chemiluminescence resonance energy transfer (CRET) and fluorescence resonance energy transfer (FRET) processes. Our results reflected CL-induced y-CDs-Ce6 system (10 μM) successfully generated reactive oxygen species (ROS, 35.93%), killed ∼90% SMMC-7721 cells in vitro, and significantly delayed tumor growth in vivo. On the basis of immunohistochemical observations of proliferating cell nuclear antigen (PCNA) and platelet/endothelial cell adhesion molecule-1 (PECAM-1 or CD31) results, we concluded that the CL-induced y-CDs-Ce6 system had excellent performance in cancer therapy. The enhanced therapeutic effect was ascribed to two pathways: a direct CRET process and another process of CRET with subsequent y-CD-mediated FRET (CRET-to-FRET).
中文翻译：
通过优化化学发光共振能量转移至光敏剂的步骤，增强治疗效果的自发光光动力疗法。
光动力疗法（PDT）的广泛应用的主要障碍是当前光敏剂的缺点以及普通外部光源的组织穿透极限。在本研究中，来自鲁米诺-H2O2-辣根过氧化物酶反应的化学发光（CL）被探索为潜在的内部光源，用于基于碳点（CD）的PDT系统的细胞内激活。为了充分利用光并提高总体PDT产量，精心选择了CD的纳米载体，CL的光和PDT剂二氢卟吩e6（Ce6），并设计形成了一个高效而统一的系统。通过有目的地调节吸收和发射光谱来合成亮黄色发射CD（y-CD），以增强化学发光共振能量转移（CRET）和荧光共振能量转移（FRET）过程中的重叠区域。我们的结果反映出CL诱导的y-CDs-Ce6系统（10μM）成功产生了活性氧（ROS，35.93％），在体外杀死了约90％的SMMC-7721细胞，并显着延迟了体内肿瘤的生长。根据增殖细胞核抗原（PCNA）和血小板/内皮细胞粘附分子1（PECAM-1或CD31）的免疫组织化学观察结果，我们得出结论，CL诱导的y-CDs-Ce6系统在癌症中具有出色的性能。治疗。增强的治疗作用归因于两个途径：
更新日期：2020-01-09
中文翻译：
通过优化化学发光共振能量转移至光敏剂的步骤，增强治疗效果的自发光光动力疗法。
光动力疗法（PDT）的广泛应用的主要障碍是当前光敏剂的缺点以及普通外部光源的组织穿透极限。在本研究中，来自鲁米诺-H2O2-辣根过氧化物酶反应的化学发光（CL）被探索为潜在的内部光源，用于基于碳点（CD）的PDT系统的细胞内激活。为了充分利用光并提高总体PDT产量，精心选择了CD的纳米载体，CL的光和PDT剂二氢卟吩e6（Ce6），并设计形成了一个高效而统一的系统。通过有目的地调节吸收和发射光谱来合成亮黄色发射CD（y-CD），以增强化学发光共振能量转移（CRET）和荧光共振能量转移（FRET）过程中的重叠区域。我们的结果反映出CL诱导的y-CDs-Ce6系统（10μM）成功产生了活性氧（ROS，35.93％），在体外杀死了约90％的SMMC-7721细胞，并显着延迟了体内肿瘤的生长。根据增殖细胞核抗原（PCNA）和血小板/内皮细胞粘附分子1（PECAM-1或CD31）的免疫组织化学观察结果，我们得出结论，CL诱导的y-CDs-Ce6系统在癌症中具有出色的性能。治疗。增强的治疗作用归因于两个途径：
京公网安备 11010802027423号