As a representative colorimetic biosnesor, paper-based LFSA have emerged as a promising and robust tool that can easily and instansly detect the presence of target biological components in food sample. Recently, LFSAs have gained a considerable attention as an alternative method for rapid diagnosis of foodborne pathogens to the conventional culture-based assays such as plate counting and PCR. One major drawback of the current LFSAs for the detection of pathogenic bacteria is the low sensitivity, limiting its practical applications in POCT. Not like many other protein-based biomarkers that are present in nM or pM range, the number of pathogenic bacteria that cause disease can be as low as few CFU/ml. Here, we review current advances in LFSAs for the detection of pathogenic bacteria in terms of chromatic agents and analyte types. Furthermore, recent approaches for signal enhancement and modifications of the LFSA architecture for multiplex detection of pathogenic bacteria are included in this review, together with the advantages and limitations of each techniques. Finally, the technological challenges and future prospect of LFSA-based POCT for the detection of pathogenic bacteria are discussed.

作为代表性的比色生物传感器，纸基LFSA已成为一种有前途且强大的工具，可以轻松，即时地检测食品样品中目标生物成分的存在。近年来，LFSA作为一种快速诊断食源性病原体的替代方法已经引起了人们的广泛关注，这是传统的基于培养物的检测方法（例如平板计数和PCR）的快速替代方法。当前用于检测病原细菌的LFSA的一个主要缺点是灵敏度低，限制了其在POCT中的实际应用。与存在于nM或pM范围内的许多其他基于蛋白质的生物标志物不同，引起疾病的病原细菌数量可以低至CFU / ml。在这里，我们从色剂和分析物类型方面回顾了LFSAs在检测病原细菌方面的最新进展。此外，这篇综述包括了信号增强和用于病原菌多重检测的LFSA架构修改的最新方法，以及每种技术的优点和局限性。最后，讨论了基于LFSA的POCT技术在病原菌检测中的技术挑战和未来前景。