Given the increasing use of gnotobiotic mouse models for deciphering the effects of human microbial communities on host biology, there is a need to develop new methods for characterizing these animals while maintaining their isolation from environmental microbes. We describe a method for performing open-circuit indirect calorimetry on gnotobiotic mice colonized with gut microbial consortia obtained from different human donors. In this illustrative case, cultured collections of gut bacterial strains were obtained from obese and lean co-twins. The approach allows microbial contributions to host energy homeostasis to be characterized.

中文翻译：

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应用间接开路量热法研究具有不同人类肠道微生物群落的生gnotobiotic小鼠的能量消耗。

鉴于越来越多地使用生gnotobiotic小鼠模型来破译人类微生物群落对宿主生物学的影响，因此需要开发新的方法来表征这些动物，同时保持它们与环境微生物的隔离。我们描述了一种方法，对从不同人类供体获得的肠道微生物聚生群定殖的gnotobiotic小鼠进行开路间接量热法。在这种说明性情况下，从肥胖和瘦双胞胎获得肠道细菌菌株的培养物。该方法使微生物对宿主能量稳态的贡献得以表征。