BACKGROUND The edible fruit Annona cherimola has previously shown many nutritional and medicinal properties. The current study evaluates the anti-cancer and anti-proliferative properties of Annona cherimola ethanolic leaf extract (AELE) on Acute Myeloid Leukemia (AML) cell lines cultured in vitro (Monomac-1 and KG-1). METHODS The anti-proliferative effect of A. cherimola ethanolic leaf extract was evaluated via cell viability assay. Its pro-apoptotic effect was assessed through Cell Death ELISA and dual Annexin V/PI staining. To further investigate the molecular mechanism by which the extract promoted apoptosis and inhibited the proliferation of the AML cells used, apoptotic protein expression was determined through western blots. Extract composition was elucidated by Gas Chromatography-Mass Spectrometry (GC-MS). RESULTS Our results showed that the treatment with A. cherimola ethanolic leaf extract exhibited an inhibitory effect on the proliferation of both cancer cell lines used in a dose- and time-dependent manner, with no toxic effects on normal mononuclear cells (MNCs) isolated from human bone marrow. This effect was mediated by DNA fragmentation and apoptosis, as revealed by Cell Death ELISA and dual Annexin V/PI staining. Western blot analysis revealed a Bax/Bcl2 dependent mechanism of apoptosis, as well as PARP cleavage, confirming the apoptotic results observed previously. These effects may be attributed to the presence of terpenes which constitute a large component of the leafy extract, as revealed via GC-MS. CONCLUSION All the data presented in our study show that the terpene-rich A. cherimola ethanolic leaf extract exhibits an anti-proliferative and pro-apoptotic effect on the AML cell lines used.

中文翻译：

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富含萜烯的番荔枝叶提取物对白血病细胞系的促凋亡作用。


背景技术可食用水果番荔枝先前已显示出许多营养和药用特性。目前的研究评估了番荔枝乙醇叶提取物 (AELE) 对体外培养的急性髓性白血病 (AML) 细胞系（Monomac-1 和 KG-1）的抗癌和抗增殖特性。方法通过细胞活力测定评价番荔枝乙醇叶提取物的抗增殖作用。通过细胞死亡 ELISA 和双膜联蛋白 V/PI 染色评估其促凋亡作用。为了进一步研究提取物促进细胞凋亡和抑制所使用的AML细胞增殖的分子机制，通过蛋白质印迹法测定了凋亡蛋白的表达。通过气相色谱-质谱法 (GC-MS) 阐明提取物成分。结果我们的结果表明，用番荔枝乙醇叶提取物处理对两种癌细胞系的增殖均表现出剂量和时间依赖性的抑制作用，而对从番荔枝中分离出的正常单核细胞（MNC）没有毒性作用。人类骨髓。细胞死亡 ELISA 和双膜联蛋白 V/PI 染色显示，这种效应是由 DNA 片段化和细胞凋亡介导的。 Western blot 分析揭示了 Bax/Bcl2 依赖性细胞凋亡机制以及 PARP 裂解，证实了之前观察到的细胞凋亡结果。正如 GC-MS 所揭示的，这些效应可能归因于萜烯的存在，萜烯构成了叶提取物的主要成分。结论 我们研究中提供的所有数据均表明，富含萜烯的番荔枝乙醇叶提取物对所用的 AML 细胞系表现出抗增殖和促凋亡作用。