当前位置: X-MOL 学术Commun. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
The vertebrate Aqp14 water channel is a neuropeptide-regulated polytransporter.
Communications Biology ( IF 5.2 ) Pub Date : 2019-12-11 , DOI: 10.1038/s42003-019-0713-y
François Chauvigné 1 , Ozlem Yilmaz 2 , Alba Ferré 1 , Per Gunnar Fjelldal 3 , Roderick Nigel Finn 1, 2 , Joan Cerdà 1
Affiliation  

Water channels (aquaporins) were originally discovered in mammals with fourteen subfamilies now identified (AQP0-13). Here we show that a functional Aqp14 subfamily phylogenetically related to AQP4-type channels exists in all vertebrate lineages except hagfishes and eutherian mammals. In contrast to the water-selective classical aquaporins, which have four aromatic-arginine constriction residues, Aqp14 proteins present five non-aromatic constriction residues and facilitate the permeation of water, urea, ammonia, H2O2 and glycerol. Immunocytochemical assays suggest that Aqp14 channels play important osmoregulatory roles in piscine seawater adaptation. Our data indicate that Aqp14 intracellular trafficking is tightly regulated by the vasotocinergic/isotocinergic neuropeptide and receptor systems, whereby protein kinase C and A transduction pathways phosphorylate highly conserved C-terminal residues to control channel plasma membrane insertion. The neuropeptide regulation of Aqp14 channels thus predates the vasotocin/vasopressin regulation of AQP2-5-6 orthologs observed in tetrapods. These findings demonstrate that vertebrate Aqp14 channels represent an ancient subfamily of neuropeptide-regulated polytransporters.

中文翻译:


脊椎动物 Aqp14 水通道是一种神经肽调节的多转运蛋白。



水通道(水通道蛋白)最初是在哺乳动物中发现的,现已确定有 14 个亚科 (AQP0-13)。在这里,我们表明,在系统发育上与 AQP4 型通道相关的功能性 Aqp14 亚家族存在于除盲鳗和真兽类哺乳动物之外的所有脊椎动物谱系中。与具有四个芳香族精氨酸收缩残基的水选择性经典水通道蛋白相比,Aqp14 蛋白具有五个非芳香族收缩残基,并促进水、尿素、氨、H2O2 和甘油的渗透。免疫细胞化学分析表明 Aqp14 通道在鱼类海水适应中发挥重要的渗透调节作用。我们的数据表明,Aqp14 细胞内运输受到血管素能/同位素能神经肽和受体系统的严格调节,由此蛋白激酶 C 和 A 转导途径磷酸化高度保守的 C 端残基以控制通道质膜插入。因此,Aqp14 通道的神经肽调节早于在四足动物中观察到的 AQP2-5-6 直向同源物的加压素/加压素调节。这些发现表明,脊椎动物 Aqp14 通道代表了神经肽调节的多转运蛋白的一个古老亚家族。
更新日期:2019-12-11
down
wechat
bug