Fluorescent nanoprobes are highly desirable toolkit for bioimaging applications. This study reports the first example for the synthesis of a nontoxic prototypical fluorescent organic compound 2‐benzo[d]thiazol‐2‐yl)‐3‐(2‐chloro‐4‐(dimethylamino)phenyl)acrylonitrile (BCA) and its entrapment into the poly[cyclotriphosphazene‐co‐polyethyleneimine] cross‐linked (PCPEI) nanospheres named as BCA@PCPEI for targeted cell imaging application. The as‐prepared BCA@PCPEI nanospheres were thoroughly characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), fourier transform infrared (FTIR), thermogravimetric analysis (TGA), and phosphorus‐31 nuclear magnetic resonance (³¹P‐NMR) analyses. The surface functional analysis of the nanospheres was performed by X‐ray photoelectron spectroscopy (XPS), which proves that the content ratios of elements belong to the precursors concentrations. The as‐prepared nanospheres displayed emission at 606 nm with bright orange fluorescence at any concentration. Moreover, the nanospheres were also less cytotoxic and maintained remarkable cell viability up to 100 μg/mL. Owing to the fluorescence with higher emission, this material has shown excellent cell imaging performance with better targeting ability to HeLa cells.

中文翻译：

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生物相容性基于荧光多胺的环磷腈杂化纳米球用于靶向细胞成像

荧光纳米探针是用于生物成像应用的非常理想的工具包。这项研究报告了无毒原型荧光有机化合物2-苯并[ d ]噻唑-2-基）-3-（2-氯-4-（二甲基氨基）苯基）丙烯腈（BCA）的合成和包埋的第一个实例进入名为BCA @ PCPEI的聚[环三磷腈-共聚乙烯亚胺]交联（PCPEI）纳米球，用于靶向细胞成像应用。制备的BCA @ PCPEI纳米球通过扫描电子显微镜（SEM），透射电子显微镜（TEM），傅立叶变换红外光谱（FTIR），热重分析（TGA）和磷31核磁共振（³¹P-NMR）分析。纳米球的表面功能分析是通过X射线光电子能谱（XPS）进行的，证明了元素的含量比属于前体浓度。所制备的纳米球在任何浓度下均显示606 nm处的发射光和亮橙色荧光。此外，纳米球的细胞毒性也较小，并保持了高达100μg/ mL的显着细胞活力。由于具有较高的发射荧光，该材料显示了出色的细胞成像性能，并且对HeLa细胞具有更好的靶向能力。