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Multifunctional BSA Scaffolds Prepared with a Novel Combination of UV‐Crosslinking Systems
Macromolecular Chemistry and Physics ( IF 2.5 ) Pub Date : 2019-11-18 , DOI: 10.1002/macp.201900378 Alina Gabriela Rusu 1 , Aurica P. Chiriac 1 , Loredana Elena Nita 1 , Liliana Mititelu‐Tartau 2 , Nita Tudorachi 1 , Alina Ghilan 1 , Daniela Rusu 1
Macromolecular Chemistry and Physics ( IF 2.5 ) Pub Date : 2019-11-18 , DOI: 10.1002/macp.201900378 Alina Gabriela Rusu 1 , Aurica P. Chiriac 1 , Loredana Elena Nita 1 , Liliana Mititelu‐Tartau 2 , Nita Tudorachi 1 , Alina Ghilan 1 , Daniela Rusu 1
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This study presents the hydrogelation of bovine serum albumin by using a natural‐derived photo‐initiator system based on riboflavin and l‐arginine that ensures the preparation of biocompatible hydrogels. Fourier transform infrared spectroscopy reveals that the synthesis conditions do not induce protein denaturation and confirms the potential UV‐crosslinking mechanism for albumin hydrogels realization. Thermal and morphological characterization indicates the formation of thermodynamically stable hydrogels with interconnected inner structure which presents a riboflavin concentration‐dependent porosity. The evolution of the hydrogel's decomposition products is analyzed by thermogravimetric analysis coupled with Fourier transform infrared spectroscopy and mass spectrometry and reveals a degradation pathway characterized by deamination of unreacted amino moieties and peptide NH groups, condensation, decomposition of the condensation products, and oxidation of N‐containing residues. The kinetics and pH‐responsive behavior of hydrogels are investigated by water uptake dynamics revealing a swelling mechanism which can be switched from a less Fickian to a non‐Fickian process by varying the crosslinker concentration. In vivo tests prove the high degree of biocompatibility of the new hydrogels, after oral administration in mice.
中文翻译：
新型BSA支架与紫外线交联系统的新型组合制备
这项研究通过使用基于核黄素和l的天然来源的光引发剂体系介绍了牛血清白蛋白的水凝胶化作用精氨酸确保生物相容性水凝胶的制备。傅里叶变换红外光谱表明，合成条件不会引起蛋白质变性，并证实了实现白蛋白水凝胶的潜在紫外线交联机理。热学和形态学特征表明具有相互联系的内部结构的热力学稳定水凝胶的形成，呈现出核黄素浓度依赖性的孔隙度。所述水凝胶的分解产物的演变是通过加上傅立叶热重量分析进行分析变换红外光谱和质谱分析和揭示了降解途径特征在于未反应的氨基部分和肽脱氨 NH 基团，缩合，缩合产物的分解以及含氮残基的氧化。通过吸水动力学研究了水凝胶的动力学和pH响应行为，揭示了一种溶胀机理，可以通过改变交联剂浓度将其从较少的Fickian转变为非Fickian的过程。在小鼠体内口服后，体内试验证明了这种新型水凝胶具有高度的生物相容性。
更新日期：2019-11-18
中文翻译：
新型BSA支架与紫外线交联系统的新型组合制备
这项研究通过使用基于核黄素和l的天然来源的光引发剂体系介绍了牛血清白蛋白的水凝胶化作用精氨酸确保生物相容性水凝胶的制备。傅里叶变换红外光谱表明，合成条件不会引起蛋白质变性，并证实了实现白蛋白水凝胶的潜在紫外线交联机理。热学和形态学特征表明具有相互联系的内部结构的热力学稳定水凝胶的形成，呈现出核黄素浓度依赖性的孔隙度。所述水凝胶的分解产物的演变是通过加上傅立叶热重量分析进行分析变换红外光谱和质谱分析和揭示了降解途径特征在于未反应的氨基部分和肽脱氨 NH 基团，缩合，缩合产物的分解以及含氮残基的氧化。通过吸水动力学研究了水凝胶的动力学和pH响应行为，揭示了一种溶胀机理，可以通过改变交联剂浓度将其从较少的Fickian转变为非Fickian的过程。在小鼠体内口服后，体内试验证明了这种新型水凝胶具有高度的生物相容性。
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