Indocyanine green (ICG) provides an advantage in the imaging of deep tumors as it can reach deeper location without being absorbed in the upper layers of biological tissues in the wavelengths, which named “therapeutic window” in the tissue engineering. Unfortunately, rapid elimination and short‐term stability in aqueous media limited its use as a fluorescence probe for the early detection of cancerous tissue. In this study, stabilization of ICG was performed by encapsulating ICG molecules into the biodegradable polymer composited with poly(l‐lactic acid) and poly(ε‐caprolactone) via a simple one‐step multiaxial electrospinning method. Different types of coaxial and triaxial structure groups were performed and compared with single polymer only groups. Confocal microscopy was used to image the encapsulated ICG (1 mg/mL) within electrospun nanofibers and in vitro ICG uptake by MIA PaCa‐2 pancreatic cancer cells. Stability of encapsulated ICG is demonstrated by the in vitro sustainable release profile in PBS (pH = 4 and 7) up to 21 days. These results suggest the potential of the ability of internalization and accommodation of ICG into the pancreatic cell cytoplasm from in vitro implanted ICG‐encapsulated multiaxial nanofiber mats. ICG‐encapsulated multilayer nanofibers may be promising for the local sustained delivery system to eliminate loss of dosage caused by direct injection of ICG‐loaded nanoparticles in systemic administration.

中文翻译：

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用于体外成像的基于吲哚菁绿的荧光聚合物纳米探针。

吲哚菁绿（ICG）在深部肿瘤的成像方面具有优势，因为它可以到达更深的位置而不会被生物组织上层吸收的波长，在组织工程中被称为“治疗窗口”。不幸的是，在水性介质中的快速消除和短期稳定性限制了它作为荧光探针用于癌组织早期检测的应用。在这项研究中，ICG 的稳定是通过将​​ ICG 分子封装到与聚（l-乳酸）和聚（ε-己内酯）通过简单的一步多轴静电纺丝方法。进行了不同类型的同轴和三轴结构组，并与单一聚合物组进行了比较。共聚焦显微镜用于对电纺纳米纤维内封装的 ICG (1 mg/mL) 和MIA PaCa-2 胰腺癌细胞体外ICG 摄取进行成像。在 PBS（pH = 4 和 7）中长达 21 天的体外可持续释放曲线证明了封装的 ICG 的稳定性。这些结果表明 ICG 具有从体外内化和容纳到胰腺细胞质中的潜力。植入ICG封装的多轴纳米纤维垫。ICG 包裹的多层纳米纤维可能有希望用于局部持续给药系统，以消除在全身给药中直接注射载有 ICG 的纳米颗粒引起的剂量损失。