Implant‐related infection (IRI) is a devastating complication in orthopedic procedures. Traditional materials used in orthopedics are susceptible to bacterial infection. In this study, we developed a copper‐bearing 316L stainless steel (316L‐Cu SS) for the prevention of IRI. This 316L‐Cu SS allowed stable and continuous release of copper ions with a rate of 5.079 ng/cm²/day. Compared with 316L stainless steel (316L SS), 316L‐Cu SS exhibited a broad‐spectrum antibacterial effect against Staphylococcus aureus, Escherichia coli, and Staphylococcus epidermidis with the bacterial reduction percentages of 95.2, 94.8, and 94.1%, respectively. The antibiofilm activity was confirmed by crystal violet assay, scanning electron microscopy, and confocal laser scanning microscopy. The in vivo antibacterial performance was tested on a rat model. When nails were treated with a low concentration of bacteria, 316L SS group exhibit a bone infection with a radiographic score of 8.9 ± 1.1 and a histological score of 10.4 ± 1.0, which were higher than 316L‐Cu SS group (1.2 ± 0.2 and 0.9 ± 0.2), indicating IRI was reduced by 316L‐Cu SS. When nails were treated with a high concentration of bacteria, IRI was also alleviated by 316L‐Cu SS. Together, these results demonstrated that 316L‐Cu SS is a promising material for preventing IRI.

中文翻译：

---

含铜 316L 不锈钢的抗菌活性用于预防种植体相关感染。

种植体相关感染 (IRI) 是骨科手术中的一种破坏性并发症。骨科中使用的传统材料容易受到细菌感染。在这项研究中，我们开发了一种含铜 316L 不锈钢 (316L-Cu SS) 来预防 IRI。这种 316L-Cu SS 允许以 5.079 ng/cm ² /天的速率稳定和连续释放铜离子。与316L不锈钢（316L SS）相比，316L-Cu SS对金黄色葡萄球菌、大肠杆菌和表皮葡萄球菌具有广谱抗菌作用细菌减少率分别为 95.2、94.8 和 94.1%。通过结晶紫测定、扫描电子显微镜和共聚焦激光扫描显微镜证实了抗生物膜活性。在大鼠模型上测试了体内抗菌性能。当指甲用低浓度细菌处理时，316L SS组表现出骨感染，放射学评分为8.9±1.1，组织学评分为10.4±1.0，高于316L-Cu SS组（1.2±0.2和0.9） ± 0.2)，表明 IRI 被 316L-Cu SS 降低。当用高浓度细菌处理指甲时，316L-Cu SS 也减轻了 IRI。总之，这些结果表明 316L-Cu SS 是一种很有前途的防止 IRI 的材料。