Hemocompatible materials for extracorporeal life support (ECLS) technology are investigated to mitigate thrombotic complications associated with this therapy. A promising solution is an omniphobic bilayer coating, tethered liquid perfluorocarbon (TLP), which utilizes an immobilized tether to anchor a mobile, liquid surface lubricant that prevents adhesion of blood components to the substrate. In this study, we investigated the effects of TLP on real‐time clot formation using thromboelastography (TEG). TLP was applied to TEG cups, utilizing perfluorodecalin (PFD) or FluorLube63 as the liquid layer, and compared to uncoated cups. Human blood (n = 10) was added to cups; and TEG parameters (R, K, α‐angle, MA, LY30, LY60) and adherent thrombus weight were assessed. TLP decreased clot amplification (α‐angle), clot strength (MA), and adherent clot weight (p < .0001). These effects were greater with FluorLube63 versus PFD (α‐angle p < .0001; MA p = .0019; clot weight p < .0001). Reaction time (R) was longer in TLP‐coated cups versus control cups with liquid lubricant added (p = .0377). Percent fibrinolysis (LY30 and LY60) was greater in the TLP versus controls at LY30 (p < .0001), and in FluoroLube63 versus controls at LY60 (p = .0021). TLP significantly altered clot formation, exerting antithrombogenic effects. This reduction in surface thrombogenicity supports TLP as a candidate for improved biocompatibility of ECLS materials, pending further validation with exposure to shear stress.

中文翻译：

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系留液体全疏水表面涂层可降低表面血栓形成、延迟凝块形成并降低体外凝块强度。

研究了用于体外生命支持 (ECLS) 技术的血液相容性材料，以减轻与该疗法相关的血栓并发症。一个有前景的解决方案是全疏水双层涂层，系链液体全氟化碳 (TLP)，它利用固定系绳来固定可移动的液体表面润滑剂，防止血液成分粘附到基材上。在这项研究中，我们使用血栓弹力图 (TEG) 研究了 TLP 对实时凝块形成的影响。TLP 应用于 TEG 杯，使用全氟萘烷 (PFD) 或 FluorLube63 作为液体层，并与未涂层的杯进行比较。人血 ( n= 10) 被添加到杯子中；评估了 TEG 参数（R、K、α 角、MA、LY30、LY60）和粘附血栓的重量。TLP 降低了凝块放大（α 角）、凝块强度 (MA) 和粘附凝块重量 ( p  < .0001)。与 PFD 相比，FluorLube63 的这些影响更大（α 角p  < .0001；MA p = .0019；凝块重量p  < .0001）。与添加液体润滑剂的对照杯相比，TLP 涂层杯的反应时间 (R) 更长 ( p = .0377)。在 LY30 时，TLP 中的纤溶百分比（LY30 和 LY60）高于对照组（p  < .0001），而 FluoroLube63 中的纤溶百分比（LY30 和 LY60）高于LY60 时的对照（p= .0021）。TLP 显着改变凝块形成，发挥抗血栓形成作用。这种表面血栓形成的减少支持 TLP 作为改善 ECLS 材料生物相容性的候选者，有待进一步验证暴露于剪切应力。