Epidural adhesion between the spinal dura and the surrounding fibrous tissue often occurs post‐laminectomy, resulting in clinical symptoms such as nerve compression and severe pain. In this study, we report a drug‐loaded double‐layered electrospun nanofiber membrane to prevent the occurrence of epidural adhesion. The nanofibers in both layers are made of a mixture of polycaprolactone (PCL) and chitosan (CS) but at different weight ratios. The bottom layer contacting to the spinal dura is loaded with meloxicam (MX) to prevent inflammation. The top layer that contacts to the fibrous tissue is doped with mitomycin‐C (MMC) to inhibit the synthesis of DNA and collagen. The two types of drugs are released from the double‐layered membrane within about 12 days. Meanwhile, the membrane can inhibit fibroblasts proliferation in vitro while show no cytotoxicity. In a rabbit laminectomy model, the double‐layered membrane can effectively prevent the epidural adhesion formation based on the adhesion scores, histological and biochemical evaluations. The combination release of MX and MMC can signally reduce the inflammation reaction and collagen I/III expression relative to the case with the membranes loaded with only either one type of the drugs. This approach offers new progresses in constructing dual drug delivery system and provides innovative barrier strategy in inhibiting epidural adhesion post‐laminectomy.

中文翻译：

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通过双层静电纺丝膜有效递送丝裂霉素 C 和美洛昔康以预防硬膜外粘连。

椎板切除术后常发生硬脊膜与周围纤维组织的硬膜外粘连，导致神经受压、剧烈疼痛等临床症状。在这项研究中，我们报告了一种载药双层电纺纳米纤维膜，以防止硬膜外粘连的发生。两层中的纳米纤维均由聚己内酯 (PCL) 和壳聚糖 (CS) 的混合物制成，但重量比不同。与脊髓硬脑膜接触的底层装有美洛昔康 (MX) 以防止炎症。与纤维组织接触的顶层掺杂有丝裂霉素 C (MMC) 以抑制 DNA 和胶原蛋白的合成。这两种药物在大约 12 天内从双层膜中释放出来。同时，该膜在体外可抑制成纤维细胞增殖，同时无细胞毒性。在兔椎板切除模型中，根据粘连评分、组织学和生化评估，双层膜可以有效防止硬膜外粘连的形成。MX 和 MMC 的组合释放可以显着降低炎症反应和胶原蛋白 I/III 的表达，相对于仅装载一种类型药物的膜的情况。该方法为构建双给药系统提供了新进展，并为抑制椎板切除术后硬膜外粘连提供了创新的屏障策略。MX 和 MMC 的组合释放可以显着降低炎症反应和胶原蛋白 I/III 的表达，相对于仅装载一种类型药物的膜的情况。该方法为构建双给药系统提供了新进展，并为抑制椎板切除术后硬膜外粘连提供了创新的屏障策略。MX 和 MMC 的组合释放可以显着降低炎症反应和胶原蛋白 I/III 的表达，相对于仅装载一种类型药物的膜的情况。该方法为构建双给药系统提供了新进展，并为抑制椎板切除术后硬膜外粘连提供了创新的屏障策略。