Hypothyroidism is an autoimmune disease associated with underactive thyroid gland. In this study, a dual effect polymeric system was designed to release Cepharanthine (CEP) to block T cell activation and Selenium (Se) to decrease the anti‐thyroid peroxidase (TPOAb) concentration in order to treat hypothyroidism. For this purpose, poly(ethylene‐vinyl acetate) (PEVA) and polyethylene glycol (PEG) nanoparticles (NPs) including CEP were synthesized by emulsion solvent evaporation method and they were loaded to polyurethane (PU)/PEG‐PUSe‐PEG block copolymer blends which were fabricated by particulate leaching technique as porous sponges. Fourier‐Transform Infrared (FTIR), Raman, and Nuclear Magnetic Resonance (NMR) analysis showed successful synthesis of PEG‐PUSe‐PEG block copolymer. A long‐term zero‐order release profile was obtained for CEP. Se release rate from matrices showed an oxidative stress‐mediated release which can be used to adjust Se amount. According to MTS results conducted by NIH 3T3 fibroblasts, both NPs and matrices have no adverse effect on cell viability. Fluorescence microscopy and SEM images confirm the MTS results. The dual release system has potential to be effectively used in long‐term treatment of hypothyroidism by addressing both auto‐immune response and hormone regulation.

中文翻译：

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一种用于治疗甲状腺功能减退症的多功能长效释放系统。

甲状腺功能减退症是一种与甲状腺功能减退相关的自身免疫性疾病。在这项研究中，设计了一种双效聚合物系统来释放 Cepharanthine (CEP) 以阻止 T 细胞活化和硒 (Se) 以降低抗甲状腺过氧化物酶 (TPOAb) 浓度以治疗甲状腺功能减退症。为此，通过乳液溶剂蒸发法合成了聚（乙烯-醋酸乙烯酯）（PEVA）和包括 CEP 的聚乙二醇（PEG）纳米粒子（NPs），并将它们负载到聚氨酯（PU）/PEG-PUSe-PEG 嵌段共聚物中通过颗粒浸出技术制造的共混物作为多孔海绵。傅里叶变换红外 (FTIR)、拉曼和核磁共振 (NMR) 分析表明 PEG-PUSe-PEG 嵌段共聚物的合成成功。获得了 CEP 的长期零级释放曲线。基质中的硒释放率显示出氧化应激介导的释放，可用于调节硒量。根据 NIH 3T3 成纤维细胞进行的 MTS 结果，NPs 和基质都对细胞活力没有不利影响。荧光显微镜和 SEM 图像证实了 MTS 结果。通过解决自身免疫反应和激素调节，双重释放系统有可能有效地用于长期治疗甲状腺功能减退症。