A current approach in bone tissue engineering is the implantation of polymeric scaffolds that promote osteoblast attachment and growth as well as biomineralization. One promising polymer is oligo[poly(ethylene glycol) fumarate] (OPF), a polyethylene glycol‐based material that is biocompatible, injectable, and biodegradable, but in its native form does not support robust bone cell attachment or growth. To address this issue, this study evaluated the osteoconductivity of bis[02‐(methacryloyloxy)ethyl] phosphate (BP) functionalized OPF hydrogels (OPF‐BP) using MC3T3‐E1 pre‐osteoblast cells, both before and after enzymatic mineralization with a calcium solution. The inclusion of negatively charged functional groups allowed for the tailored uptake and release of calcium, while also altering the mechanical properties and surface topography of the hydrogel surface. In cell culture, OPF‐BP hydrogels with 20 and 30% (w/w) BP optimized osteoblast attachment, proliferation, and differentiation after a 21‐day in vitro period. In addition, the OPF‐BP30 treatment, when mineralized with calcium, exhibited a 128% increase in osteocalcin expression when compared with the non‐mineralized treatment. These findings suggest that phosphate functionalization and enzymatic calcium mineralization can act synergistically to enhance the osteoconductivity of OPF hydrogels, making this processed material an attractive candidate for bone tissue engineering applications.

中文翻译：

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磷酸盐功能化和酶钙矿化协同增强低聚[聚（乙二醇）富马酸酯]水凝胶骨传导性，用于骨组织工程。


目前骨组织工程的方法是植入聚合物支架，促进成骨细胞附着和生长以及生物矿化。一种有前途的聚合物是低聚[聚乙二醇富马酸酯]（OPF），这是一种基于聚乙二醇的材料，具有生物相容性、可注射性和可生物降解性，但其天然形式不支持牢固的骨细胞附着或生长。为了解决这个问题，本研究使用 MC3T3-E1 前成骨细胞评估了双[02-(甲基丙烯酰氧基)乙基]磷酸酯 (BP) 功能化 OPF 水凝胶 (OPF-BP) 的骨传导性，在钙酶矿化前后进行了评估。解决方案。包含带负电的官能团可以定制钙的吸收和释放，同时也改变水凝胶表面的机械性能和表面形貌。在细胞培养中，含有 20% 和 30%（w/w）BP 的 OPF-BP 水凝胶在体外 21 天后优化了成骨细胞的附着、增殖和分化。此外，与非矿化治疗相比，OPF-BP30 治疗在用钙矿化时，骨钙素表达增加了 128%。这些发现表明，磷酸盐功能化和酶促钙矿化可以协同作用，增强 OPF 水凝胶的骨传导性，使这种加工材料成为骨组织工程应用的有吸引力的候选者。