Functional comparison of beating cardiomyocytes differentiated from umbilical cord-derived mesenchymal/stromal stem cells and human foreskin-derived induced pluripotent stem cells.,Journal of Biomedical Materials Research Part A

当前位置： X-MOL 学术 › J. Biomed. Mater. Res. Part A › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Functional comparison of beating cardiomyocytes differentiated from umbilical cord-derived mesenchymal/stromal stem cells and human foreskin-derived induced pluripotent stem cells.
Journal of Biomedical Materials Research Part A ( IF 3.9 ) Pub Date : 2019-11-22 , DOI: 10.1002/jbm.a.36831
Pallavi Pushp _{1,

2} , Bijayalaxmi Sahoo ₁ , Frederico C Ferreira ₃ , Joaquim M Sampaio Cabral ₃ , Ana Fernandes-Platzgummer ₃ , Mukesh K Gupta ₁

Affiliation

In recent years, stem cell‐based therapies shown to have promising effects on the clinical management of ischemic heart disease. Moreover, stem cells differentiation into cardiomyocytes (CMs) can overcome the cell source limitations. The current research involves the isolation and expansion of mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPSCs), their differentiation into CMs and subsequent construction of tissue‐engineered myocardium supported by random and aligned polycaprolactone (PCL) nanofibrous matrices (av. dia: 350–850 nm). Umbilical cord matrix (UCM)‐derived MSCs were isolated successfully by routine enzymatic digestion and a nonenzymatic explant culture method and characterized by their morphology, differentiation into different lineages, and surface marker expression. Treatment of UCM‐derived MSCs with 5‐azacytidine (5 μM) induced their differentiation into putative cardiac cells, as revealed by the expression of cardiac‐specific troponin T (cTnT), smooth muscles actin, myogenin (MYOG), smoothelin, cardiac α‐actin genes and cTnT, α‐actinin proteins by RT‐PCR and immunocytochemistry, respectively. However, no beating cells were observed in differentiated MSCs. On the other hand, adult human foreskin‐derived iPSCs cultured on Matrigel™—coated aligned PCL nanofibrous matrices showed anisotropic behavior along the PCL nanofibers and, upon differentiation, expressed cardiac‐specific cTnT (23.34 vs. 32.55%) proteins and showed more synchronized beating than those differentiated on Matrigel™—coated tissue culture coated polystyrene surfaces. Moreover, aligned PCL nanofibers are able to promote cells orientation parallel to the fibers, thus providing an effective way to control anisotropic nature under in vitro condition.

中文翻译：

从脐带来源的间充质/基质干细胞和人包皮来源的诱导多能干细胞分化的跳动心肌细胞的功能比较。

近年来，基于干细胞的疗法显示出对缺血性心脏病的临床管理具有良好的效果。此外，干细胞分化为心肌细胞 (CMs) 可以克服细胞来源的限制。目前的研究涉及间充质干细胞 (MSCs) 和诱导多能干细胞 (iPSCs) 的分离和扩增、它们向 CMs 的分化以及随后由随机排列的聚己内酯 (PCL) 纳米纤维基质支持的组织工程心肌的构建。直径：350–850 纳米）。通过常规酶消化和非酶外植体培养方法成功分离了脐带基质 (UCM) 衍生的 MSC，并对其形态、分化为不同谱系和表面标记表达进行了表征。如心脏特异性肌钙蛋白 T (cTnT)、平滑肌肌动蛋白、肌细胞生成素 (MYOG)、平滑蛋白、心脏 α 的表达所揭示的，用 5-氮杂胞苷 (5 μM) 处理 UCM 衍生的 MSC通过 RT-PCR 和免疫细胞化学分别检测肌动蛋白基因和 cTnT、α-肌动蛋白。然而，在分化的 MSCs 中没有观察到跳动的细胞。另一方面，在 Matrigel™ 上培养的成人包皮来源的 iPSCs - 涂层排列的 PCL 纳米纤维基质显示出沿 PCL 纳米纤维的各向异性行为，并且在分化后，表达心脏特异性 cTnT（23.34 对 32.55%）蛋白并显示出更同步的比那些在 Matrigel™ 上分化的打浆 - 涂层组织培养涂层聚苯乙烯表面。而且，

更新日期：2019-11-22

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11