The aim of this study was to investigate the characteristic gene expression profile and localization of peri‐implant connective tissue (PICT) compared with those of periodontal connective tissue (PCT) and oral mucosal connective tissue (OMCT). Upper first molar of 5‐week‐old rats were extracted and titanium implant were placed for PICT group. PCT and OMCT were used as control. Laser microdissected connective tissue at 4 weeks used for microarray analysis. The expression and localization of selected genes were confirmed by quantitative real‐time PCR (qRT‐PCR) and immunohistochemistry. Approximately, 1000 genes of upregulated and downregulated in PICT compared with PCT and OMCT were recognized. Based on the results of microarray analysis and qRT‐PCR were demonstrated lipopolysaccharide binding protein (Lbp) as a specific upregulated gene and superoxide dismutase 3 (Sod3) as a specific downregulated gene in PICT. Immunoreaction of LBP and F4/80 as macrophage marker localized to subepithelial and implant facing connective tissue in PICT. SOD3 expression was not observed in PICT, reactive oxygen species, a target of superoxide dismutase, was strongly and locally expressed in all three tissues. Our data suggested that the upregulation of Lbp and downregulation of Sod3 are as characteristic gene expression pattern in PICT.

中文翻译：

---

大鼠种植体周围结缔组织中 Lbp 和 Sod3 基因表达的特征调控[J].

本研究的目的是研究与牙周结缔组织 (PCT) 和口腔黏膜结缔组织 (OMCT) 相比，种植体周围结缔组织 (PICT) 的特征基因表达谱和定位。PICT组拔除5周龄大鼠上第一磨牙并置入钛种植体。PCT和OMCT用作对照。用于微阵列分析的 4 周激光显微切割结缔组织。所选基因的表达和定位通过实时定量 PCR (qRT-PCR) 和免疫组织化学进行确认。与PCT和OMCT相比，PICT中大约有1000个上调和下调基因被识别。基于微阵列分析和 qRT-PCR 的结果证明脂多糖结合蛋白 ( Lbp) 作为PICT 中的特定上调基因和超氧化物歧化酶 3 ( Sod3 ) 作为特定下调基因。LBP 和 F4/80 作为巨噬细胞标记的免疫反应，定位于 PICT 中的上皮下和面向结缔组织的植入物。在 PICT 中未观察到 SOD3 表达，超氧化物歧化酶的靶标活性氧在所有三种组织中均强烈且局部表达。我们的数据表明Lbp的上调和Sod3 的下调是PICT中的特征基因表达模式。