Osteoconductive hydrogels can be fabricated by incorporating necessary growth factors and bioactive particles or simply utilizing the ability of the hydrogel itself to induce bone regeneration. The osteogenic inductive potential of the bioactive glass microparticles (BG MPs) has been well‐studied. However, the role of the hydrogel embedding the BG MPs on the osteogenic differentiation of the encapsulated stem cells has not been well established. Moreover, it has been reported that the dental pulp stem cell (DPSC) has the capability of regenerating the craniofacial bone tissue when receiving the necessary osteogenic signals from the microenvironment. In the current study, we have fabricated a composite hydrogel based on alginate and Matrigel containing BG MPs and evaluated the role of the BG MPs and Matrigel on osteogenic differentiation of the encapsulated DPSCs. Our results confirmed that presence of Matrigel enhances the osteogenic differentiation of the DPSCs regardless of the decrease in elasticity of the hydrogel in presence of the BG MPs. This strategy of modifying the microenvironment can be a promising approach for craniofacial bone tissue engineering.

中文翻译：

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用于人牙髓干细胞成骨分化的生物活性玻璃水凝胶递送系统。

骨传导水凝胶可以通过加入必要的生长因子和生物活性颗粒或简单地利用水凝胶本身诱导骨再生的能力来制造。生物活性玻璃微粒（BG MPs）的成骨诱导潜力已得到充分研究。然而，嵌入 BG MP 的水凝胶对封装干细胞的成骨分化的作用尚未确定。此外，据报道，当从微环境接收必要的成骨信号时，牙髓干细胞 (DPSC) 具有再生颅面骨组织的能力。在目前的研究中，我们已经制造了一种基于藻酸盐和含有 BG MP 的基质胶的复合水凝胶，并评估了 BG MP 和基质胶对封装的 DPSCs 成骨分化的作用。我们的结果证实，无论水凝胶在 BG MP 存在下的弹性是否降低，Matrigel 的存在都会增强 DPSCs 的成骨分化。这种改变微环境的策略可能是颅面骨组织工程的一种有前途的方法。