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Evaluation of effectiveness of 45S5 bioglass doped with niobium for repairing critical-sized bone defect in in vitro and in vivo models.
Journal of Biomedical Materials Research Part A ( IF 3.9 ) Pub Date : 2019-11-07 , DOI: 10.1002/jbm.a.36826
Lucas P L de Souza 1 , João H Lopes 2 , Filipe V Ferreira 3 , Richard A Martin 4 , Celso A Bertran 5 , José A Camilli 1
Affiliation  

Here, we investigated the biocompatibility of a bioactive sodium calcium silicate glass containing 2.6 mol% Nb2O5 (denoted BGPN2.6) and compare the results with the archetypal 45S5 bioglass. The glass bioactivity was tested using a range of in vitro and in vivo experiments to assess its suitability for bone regeneration applications. in vitro studies consisted of assessing the cytocompatibility of the BGPN2.6 glass with bone‐marrow‐derived mesenchymal stem cells (BM‐MSCs). Systemic biocompatibility was verified by means of the quantification of biochemical markers and histopathology of liver, kidneys, and muscles. The glass genotoxicity was assessed using the micronucleus test. The regeneration of a calvarial defect was assessed using both qualitative and quantitative analysis of three‐dimensional microcomputed tomography images. The BGPN2.6 glass was not cytotoxic to BM‐MSCs. It is systemically biocompatible causing no signs of damage to high metabolic and excretory organs such as the liver and kidneys. No mutagenic potential was observed in the micronucleus test. MicroCT images showed that BGPN2.6 was able to nearly fully regenerate a critical‐sized calvarial defect and was far superior to standard 45S5 Bioglass. Defects filled with BGPN2.6 glass showed over 90% coverage compare to just 66% for 45S5 Bioglass. For one animal the defect was completely filled in 8 weeks. These results clearly show that Nb‐containing bioactive glasses are a safe and effective biomaterial for bone replacement.

中文翻译:

在体外和体内模型中评估掺铌 45S5 生物玻璃修复临界尺寸骨缺损的有效性。

在这里,我们研究了含有 2.6 mol% Nb 2 O 5的生物活性钠钙硅酸盐玻璃的生物相容性(表示为BGPN2.6)并将结果与​​原型45S5生物玻璃进行比较。使用一系列体外和体内实验测试玻璃生物活性,以评估其对骨再生应用的适用性。体外研究包括评估 BGPN2.6 玻璃与骨髓间充质干细胞 (BM-MSC) 的细胞相容性。全身生物相容性通过肝脏、肾脏和肌肉的生化标志物和组织病理学的量化来验证。使用微核试验评估玻璃遗传毒性。使用三维显微计算机断层扫描图像的定性和定量分析评估颅骨缺损的再生。BGPN2.6 玻璃对 BM-MSC 没有细胞毒性。它具有全身生物相容性,不会对肝脏和肾脏等高代谢和排泄器官造成损害。在微核试验中未观察到诱变潜力。MicroCT 图像显示 BGPN2.6 能够几乎完全再生临界尺寸的颅骨缺损,并且远远优于标准的 45S5 生物玻璃。填充 BGPN2.6 玻璃的缺陷显示超过 90% 的覆盖率,而 45S5 生物玻璃仅为 66%。对于一只动物,缺损在 8 周内完全填补。这些结果清楚地表明,含铌生物活性玻璃是一种安全有效的骨置换生物材料。6 能够几乎完全再生临界尺寸的颅骨缺损,并且远远优于标准的 45S5 生物玻璃。填充 BGPN2.6 玻璃的缺陷显示超过 90% 的覆盖率,而 45S5 生物玻璃仅为 66%。对于一只动物,缺损在 8 周内完全填补。这些结果清楚地表明,含铌生物活性玻璃是一种安全有效的骨置换生物材料。6 能够几乎完全再生临界尺寸的颅骨缺损,并且远远优于标准的 45S5 生物玻璃。填充 BGPN2.6 玻璃的缺陷显示超过 90% 的覆盖率,而 45S5 生物玻璃仅为 66%。对于一只动物,缺损在 8 周内完全填补。这些结果清楚地表明,含铌生物活性玻璃是一种安全有效的骨置换生物材料。
更新日期:2019-11-07
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