Osteochondral repair requires the induction of both articular cartilage and subchondral bone development, necessitating the presentation of multiple tissue‐specific cues for these highly distinct tissues. To provide a singular hydrogel system for the repair of either tissue type, we have developed biofunctionalized, mesenchymal stem cell‐laden hydrogels that can present in situ biochemical cues for either chondrogenesis or osteogenesis by simple click modification of a crosslinker, poly(glycolic acid)‐poly(ethylene glycol)‐poly(glycolic acid)‐di(but‐2‐yne‐1,4‐dithiol) (PdBT). After modifying PdBT with either cartilage‐specific biomolecules (N‐cadherin peptide, chondroitin sulfate) or bone‐specific biomolecules (bone marrow homing peptide 1, glycine‐histidine‐lysine peptide), the biofunctionalized, PdBT‐crosslinked hydrogels can selectively promote the desired bone‐ or cartilage‐like matrix synthesis and tissue‐specific gene expression, with effects dependent on both biomolecule selection and concentration. Our findings establish the versatility of this click functionalized hydrogel system as well as its ability to promote in vitro development of osteochondral tissue phenotypes.

中文翻译：

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用于骨软骨组织工程的功能化、组织特异性水凝胶。

骨软骨修复需要诱导关节软骨和软骨下骨发育，需要为这些高度不同的组织提供多种组织特异性线索。为了提供用于修复任一组织类型的单一水凝胶系统，我们开发了生物功能化的、间充质干细胞负载的水凝胶，通过对交联剂、聚（乙醇酸） -聚（乙二醇）-聚（乙醇酸）-二（丁-2-炔-1,4-二硫醇）（PdBT）。用软骨特异性生物分子（N-钙粘蛋白肽、硫酸软骨素）或骨特异性生物分子（骨髓归巢肽 1、甘氨酸-组氨酸-赖氨酸肽）修饰 PdBT 后，PdBT 交联的水凝胶可以选择性地促进所需的骨或软骨样基质合成和组织特异性基因表达，其效果取决于生物分子的选择和浓度。我们的研究结果证实了这种点击功能化水凝胶系统的多功能性及其促进骨软骨组织表型体外发育的能力。