Automated matrix deposition for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is crucial for producing reproducible analyte ion signals. Here we report an innovative method employing an automated immersion apparatus, which enables a robust matrix deposition within 5 minutes and with scalable throughput by using MAPS matrix and non-polar solvents. MSI results received from mouse heart and rat brain tissues were qualitatively similar to those from nozzle sprayed samples with respect to peak number and quality of the ion images. Overall, the immersion-method enables a fast and careful matrix deposition and has the future potential for implementation in clinical tissue diagnostics.

中文翻译：

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通过基于旋转的基质浸入法进行快速，可重现的样品制备，并在质谱成像中获得可靠的结果。

用于基质辅助激光解吸/电离（MALDI）质谱成像（MSI）的自动基质沉积对于产生可重现的分析物离子信号至关重要。在这里，我们报告了一种采用自动浸没设备的创新方法，该方法通过使用MAPS基质和非极性溶剂，可在5分钟内实现可靠的基质沉积，并具有可扩展的通量。就离子图像的峰值和质量而言，从小鼠心脏和大鼠脑组织获得的MSI结果在质量上与从喷嘴喷雾样品获得的结果相似。总体而言，浸没法可实现快速，仔细的基质沉积，并具有在临床组织诊断中实施的未来潜力。