Bond-selective transient phase imaging via sensing of the infrared photothermal effect.,Light: Science & Applications

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Bond-selective transient phase imaging via sensing of the infrared photothermal effect.
Light: Science & Applications ( IF 20.6 ) Pub Date : 2019-12-11 , DOI: 10.1038/s41377-019-0224-0
Delong Zhang _{1,

2} , Lu Lan ₁ , Yeran Bai _{1,

3,

4} , Hassaan Majeed ₅ , Mikhail E Kandel ₆ , Gabriel Popescu _{5,

6} , Ji-Xin Cheng _{1,

7,

8}

Affiliation

Phase-contrast microscopy converts the phase shift of light passing through a transparent specimen, e.g., a biological cell, into brightness variations in an image. This ability to observe structures without destructive fixation or staining has been widely utilized for applications in materials and life sciences. Despite these advantages, phase-contrast microscopy lacks the ability to reveal molecular information. To address this gap, we developed a bond-selective transient phase (BSTP) imaging technique that excites molecular vibrations by infrared light, resulting in a transient change in phase shift that can be detected by a diffraction phase microscope. By developing a time-gated pump-probe camera system, we demonstrate BSTP imaging of live cells at a 50 Hz frame rate with high spectral fidelity, sub-microsecond temporal resolution, and sub-micron spatial resolution. Our approach paves a new way for spectroscopic imaging investigation in biology and materials science.

中文翻译：

通过红外光热效应传感的键选择性瞬态相位成像。

相衬显微镜将通过透明样本（例如生物细胞）的光的相移转换为图像中的亮度变化。这种无需破坏性固定或染色即可观察结构的能力已广泛用于材料和生命科学中的应用。尽管有这些优点，但相差显微镜仍缺乏揭示分子信息的能力。为了解决这一差距，我们开发了一种键选择性瞬态相 (BSTP) 成像技术，该技术通过红外光激发分子振动，从而导致相移的瞬态变化，可以通过衍射相位显微镜检测到。通过开发时间门控泵探头相机系统，我们展示了以 50 Hz 帧速率对活细胞进行 BSTP 成像，具有高光谱保真度、亚微秒时间分辨率、和亚微米空间分辨率。我们的方法为生物学和材料科学中的光谱成像研究开辟了一条新途径。

更新日期：2019-12-11

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