Ambient ionization mass spectrometry (AIMS) has grown into a group of emerging analytical techniques that allow rapid, real-time, high-throughput, in situ, and in vivo analysis in many scientific fields including biomedicine, pharmaceuticals, and forensic sciences. While dozens of AIMS techniques have been introduced over the past two decades, their broad commercial and industrial use is still restricted by multiple challenges. In this Perspective, we discuss the most relevant technical challenges facing AIMS, i.e., reproducibility, quantitative ability, molecular coverage, sensitivity, and data complexity, and scientists' recent attempts to overcome these hurdles. Furthermore, we present future directions of AIMS from our perspective, including the necessity that efforts should be made to unravel blind biomolecules in routine analysis, the construction of a data depository for AIMS users, the full automation of pipelines for prospect integration in a robotic laboratory, the movement toward on-site tests, and the expansion of outreach to motivate government officials in policymaking. We anticipate that, with progress in these critical but immature areas, AIMS technology will keep evolving to become a more robust and user-friendly set of technologies and, consequently, be translated into everyday life practice.

中文翻译：

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如今和明天的环境电离质谱：迎接挑战和机遇。

环境电离质谱（AIMS）已成长为一组新兴的分析技术，可在包括生物医学，药物和法医科学在内的许多科学领域中进行快速，实时，高通量的原位和体内分析。尽管在过去的二十年中已经引入了数十种AIMS技术，但是它们的广泛商业和工业应用仍然受到多重挑战的限制。在本《观点》中，我们讨论了AIMS面临的最相关的技术挑战，即再现性，定量能力，分子覆盖率，灵敏度和数据复杂性，以及科学家最近为克服这些障碍而进行的尝试。此外，我们从我们的角度提出了AIMS的未来发展方向，包括在常规分析中必须努力揭开盲目的生物分子的必要性，面向AIMS用户的数据存储库的建设，用于机器人实验室中的潜在客户集成的管道的完全自动化，向现场测试的发展以及扩大范围以激励政府官员进行决策。我们预计，随着在这些关键但不成熟的领域中取得进展，AIMS技术将不断发展成为更加健壮和用户友好的技术，因此将被转化为日常生活实践。