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Factors regulating the differential uptake of persistent organic pollutants in cucurbits and non-cucurbits
Journal of Plant Physiology ( IF 4.0 ) Pub Date : 2020-02-01 , DOI: 10.1016/j.jplph.2019.153094
Aya Iwabuchi 1 , Nonoka Katte 2 , Mizuki Suwa 1 , Junya Goto 1 , Hideyuki Inui 3
Affiliation  

Contamination with persistent organic pollutants (POPs) has become a worldwide concern owing to their the toxicity to humans and wildlife. Pumpkin, cucumber, and squash (Cucurbitaceae) accumulate POPs in their shoots in concentrations higher than those in non-cucurbits; to elucidate the underlying molecular mechanisms of this accumulation, POP transporters were analyzed in the xylem sap of cucurbits and non-cucurbits. The 17-kDa xylem sap proteins detected in all cucurbits but not in non-cucurbits readily bound polychlorinated biphenyl (PCB) in all tested cucurbits, except in cucumber and loofah, and to dieldrin in all tested cucurbits. Ten genes encoding major latex-like proteins (MLPs) responsible for the accumulation of PCBs in zucchini plants were cloned from cucurbits. Phylogenetic analysis using MLP sequences identified two separate clades, one containing Cucurbitaceae MLPs and the other containing those of non-cucurbit members. Recombinant MLPs bound PCB and dieldrin. Western blotting with anti-MLP antibodies identified translocatable and non-translocatable MLPs between root and stem xylem vessels. Translocation of MLPs from the root to stem xylem vessels and POP-binding ability of MLPs are important for selective accumulation of MLPs in cucurbits. This study provides basic knowledge about phytoremediation through overexpression of MLP genes and for breeding cucurbits that accumulate less contaminants.

中文翻译:

调节葫芦科植物和非葫芦科植物中持久性有机污染物差异吸收的因素

由于持久性有机污染物(POPs)对人类和野生动物的毒性,其污染已成为全球关注的问题。南瓜、黄瓜和南瓜(葫芦科)在其枝条中积累的持久性有机污染物的浓度高于非葫芦科植物;为了阐明这种积累的潜在分子机制,在葫芦科植物和非葫芦科植物的木质部汁液中分析了 POP 转运蛋白。在所有葫芦科植物中检测到的 17-kDa 木质部汁液蛋白,但不是在非葫芦科植物中检测到的,在所有测试的葫芦科植物中,除黄瓜和丝瓜络外,容易与多氯联苯 (PCB) 结合,并在所有测试的葫芦科植物中与狄氏剂结合。从葫芦科植物中克隆了十个编码主要乳胶样蛋白 (MLP) 的基因,这些基因负责西葫芦植物中 PCB 的积累。使用 MLP 序列的系统发育分析确定了两个独立的进化枝,一种含有葫芦科 MLP,另一种含有非葫芦科成员的 MLP。重组 MLP 结合 PCB 和狄氏剂。使用抗 MLP 抗体进行蛋白质印迹鉴定了根和茎木质部血管之间的易位和非易位 MLP。MLPs 从根部转移到茎木质部血管和 MLPs 的 POP 结合能力对于 MLPs 在葫芦中的选择性积累很重要。该研究提供了有关通过 MLP 基因过表达进行植物修复和培育积累较少污染物的葫芦的基本知识。MLPs 从根部转移到茎木质部血管和 MLPs 的 POP 结合能力对于 MLPs 在葫芦中的选择性积累很重要。该研究提供了有关通过 MLP 基因过表达进行植物修复和培育积累较少污染物的葫芦的基本知识。MLPs 从根部转移到茎木质部血管和 MLPs 的 POP 结合能力对于 MLPs 在葫芦中的选择性积累很重要。该研究提供了有关通过 MLP 基因过表达进行植物修复和培育积累较少污染物的葫芦的基本知识。
更新日期:2020-02-01
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