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Aspergillus niger enhances oxalate production as a response to phosphate deficiency induced by aluminium(III).
Journal of Inorganic Biochemistry ( IF 3.8 ) Pub Date : 2019-12-11 , DOI: 10.1016/j.jinorgbio.2019.110961 Filip Polák 1 , Martin Urík 2 , Marek Bujdoš 2 , Peter Matúš 2
Journal of Inorganic Biochemistry ( IF 3.8 ) Pub Date : 2019-12-11 , DOI: 10.1016/j.jinorgbio.2019.110961 Filip Polák 1 , Martin Urík 2 , Marek Bujdoš 2 , Peter Matúš 2
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This paper investigates Aspergillus niger's behaviour in the presence of mobile Al3+ species by evaluating the changes in oxalate exudation at various aluminium contents. When the fungus was exposed to Al3+, no significant changes in oxalate production were observed until 100 mg.L-1 aluminium was reached resulting in oxalate production decrease by 18.2%. By stripping the culture medium completely of phosphate, even more prominent decrease by 34.8% and 67.1% at 10 and 100 mg.L-1 aluminium was observed, respectively, indicating the phosphate's significance instead of Al3+ in oxalate production. Our results suggest that the low phosphate bioavailability, which most likely resulted from its interaction with Al3+, stimulated the overproduction of oxalate by A. niger. Furthermore, when the fungus was incubated in aluminium-free media supplemented with 0.1 mM of phosphate, oxalate production increased up to 281.5 μmol.g-1, while at 1.85 mM of available phosphate only 80.7 μmol.g-1 of oxalate was produced. This indicates that oxalic acid is produced by fungus not as a mean to detoxify aluminium, but as an attempt to gain access to additional phosphate.
中文翻译：
黑曲霉可增强草酸盐的产生，以应对铝（III）引起的磷酸盐缺乏。
本文通过评估各种铝含量下草酸盐渗出的变化，研究了黑曲霉在可移动的Al3 +物种存在下的行为。当真菌暴露于Al3 +时，直到达到100 mg.L-1铝，草酸盐的产量均未观察到显着变化，导致草酸盐的产量降低了18.2％。通过完全去除磷酸盐的培养基，在10和100 mg.L-1铝时，分别下降了34.8％和67.1％，这表明磷酸盐在草酸盐生产中代替了Al3 +发挥了重要作用。我们的结果表明，低磷酸盐的生物利用度很可能是由其与Al3 +的相互作用引起的，它刺激了黑曲霉的草酸盐过分生产。此外，当将真菌在补充有0.1 mM磷酸盐的无铝培养基中孵育时，草酸盐的产量增加到281.5μmol.g-1，而在1.85 mM的可用磷酸盐中，仅产生80.7μmol.g-1的草酸盐。这表明草酸是由真菌产生的，并不是将铝解毒的手段，而是试图获得更多磷酸盐的手段。
更新日期：2019-12-11
中文翻译：
黑曲霉可增强草酸盐的产生，以应对铝（III）引起的磷酸盐缺乏。
本文通过评估各种铝含量下草酸盐渗出的变化，研究了黑曲霉在可移动的Al3 +物种存在下的行为。当真菌暴露于Al3 +时，直到达到100 mg.L-1铝，草酸盐的产量均未观察到显着变化，导致草酸盐的产量降低了18.2％。通过完全去除磷酸盐的培养基，在10和100 mg.L-1铝时，分别下降了34.8％和67.1％，这表明磷酸盐在草酸盐生产中代替了Al3 +发挥了重要作用。我们的结果表明，低磷酸盐的生物利用度很可能是由其与Al3 +的相互作用引起的，它刺激了黑曲霉的草酸盐过分生产。此外，当将真菌在补充有0.1 mM磷酸盐的无铝培养基中孵育时，草酸盐的产量增加到281.5μmol.g-1，而在1.85 mM的可用磷酸盐中，仅产生80.7μmol.g-1的草酸盐。这表明草酸是由真菌产生的，并不是将铝解毒的手段，而是试图获得更多磷酸盐的手段。
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