Acellular fish skin (ACS) has emerged as a dermal substitute used to promote wound healing with decreased scar formation and pain relief that may be due to polyunsaturated fatty acid (PUFA) content. However, the PUFA content of ACS is still unknown. The aim of this study was to compare the total fatty acids and lipid profiles of ACS to two bovine-based grafts and standard of care human cadaver skin (HCS). Furthermore, there was also the goal to assess the capability of ACS lipid content to enhance wound healing. The fatty acid analysis was performed with GC-FID, and an LC-MS untargeted method was developed in order to the analyse the lipid profiles of the grafts was. The enhancement of wound healing by the ACS extract was investigated in vitro on HaCat cells. Our results showed that ACS had the highest content of PUFA (27.0 ± 1.43% of their total fatty acids), followed by HCS (20.6 ± 3.9%). The two grafts of bovine origin presented insignificant PUFA amounts. The majority of the PUFAs found in ACS were omega-3, and in HCS, they were omega-6. The untargeted lipidomics analysis demonstrated that ACS grafts were characterized by phosphatidylcholine containing either 20:5 or 22:6 omega-3 PUFA. The ACS lipid extract increased the HaCat cells migration and enhanced wound closure 4 hr earlier versus control. Our study demonstrated that ACS has a lipid profile that is distinct from other wound healing grafts, that PUFAs are maintained in ACS post-processing as phosphatidylcholine, and that ACS lipid content influences wound healing properties.

中文翻译：

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伤口愈合移植物：Omega-3脂肪酸脂质含量将无细胞大西洋鳕鱼皮的脂质特性与传统的真皮替代品区分开。

无细胞鱼皮（ACS）已作为一种皮肤替代品出现，用于促进伤口愈合，并减少了疤痕的形成并减轻了疼痛，这可能是由于多不饱和脂肪酸（PUFA）含量所致。但是，ACS的PUFA含量仍然未知。这项研究的目的是比较两种牛基移植物和标准护理人尸皮肤（HCS）的ACS的总脂肪酸和脂质谱。此外，还有一个目标是评估ACS脂质含量增强伤口愈合的能力。用GC-FID进行脂肪酸分析，并开发出LC-MS非靶向方法以分析移植物的脂质分布。体外在HaCat细胞上研究了ACS提取物对伤口愈合的促进作用。我们的结果表明ACS具有最高的PUFA含量（27.0±1）。其总脂肪酸的43％），其次是HCS（20.6±3.9％）。两种源自牛的移植物的PUFA含量均不显着。ACS中发现的大多数PUFA是omega-3，而在HCS中则是omega-6。非靶向脂质组学分析表明，ACS移植物的特征是含有20：5或22：6 omega-3 PUFA的磷脂酰胆碱。ACS脂质提取物比对照提早4小时增加了HaCat细胞的迁移并增强了伤口闭合。我们的研究表明，ACS具有不同于其他伤口愈合移植物的脂质特性，PUFA在磷脂酰胆碱的后处理过程中得以维持，并且ACS脂质含量影响伤口的愈合性能。ACS中发现的大多数PUFA是omega-3，而在HCS中则是omega-6。非靶向脂质组学分析表明，ACS移植物的特征是含有20：5或22：6 omega-3 PUFA的磷脂酰胆碱。ACS脂质提取物比对照提早4小时增加了HaCat细胞的迁移并增强了伤口闭合。我们的研究表明，ACS具有不同于其他伤口愈合移植物的脂质特性，PUFA在磷脂酰胆碱的后处理过程中得以维持，并且ACS脂质含量影响伤口愈合特性。ACS中发现的大多数PUFA是omega-3，而在HCS中则是omega-6。非靶向脂质组学分析表明，ACS移植物的特征是含有20：5或22：6 omega-3 PUFA的磷脂酰胆碱。ACS脂质提取物比对照提早4小时增加了HaCat细胞的迁移并增强了伤口闭合。我们的研究表明，ACS具有不同于其他伤口愈合移植物的脂质特性，PUFA在磷脂酰胆碱的后处理过程中得以维持，并且ACS脂质含量影响伤口愈合特性。ACS脂质提取物比对照提早4小时增加了HaCat细胞的迁移并增强了伤口闭合。我们的研究表明，ACS具有不同于其他伤口愈合移植物的脂质特性，PUFA在磷脂酰胆碱的后处理过程中得以维持，并且ACS脂质含量影响伤口愈合特性。ACS脂质提取物比对照提早4小时增加了HaCat细胞的迁移并增强了伤口闭合。我们的研究表明，ACS具有不同于其他伤口愈合移植物的脂质特性，PUFA在磷脂酰胆碱的后处理过程中得以维持，并且ACS脂质含量影响伤口愈合特性。