An efficient ultrasound enhanced matrix solid-phase dispersion micro-extraction method was established for the extraction and determination of multiple ingredients (psoralenoside, isopsoralenoside, psoralen, isopsoralen, bavachin, psoralidin, isobavachalcone and bakuchiol) from Fructus Psoraleae applying ultra-high-performance liquid chromatography. The experimental factors affecting the extraction efficiency, including extraction solvent concentration, extraction solvent volume and ultrasound time, were optimized by the design of experiments methodology. Central composite design was applied in this optimization approach as a quadratic model. Under optimum conditions, good linear regression (r² > 0.9999) and recovery (96.8%–104%, RSD<3.73%) for targets were attained. In addition, the intra-day of analytes was below 4.42% and inter-day precision of analytes was below 2.08%. This proposed method only required small amounts of reagent (0.5 mL) and materials usage (20 mg). Thus, the present study applied the efficient method to exhibit fast preparation and determination of eight ingredients of continents in Fructus Psoraleae.

建立了一种高效的超声增强基质固相分散微萃取方法，采用超高效液相色谱法从s骨果中提取和测定多种成分（补骨脂甙，异补骨脂甙，补骨脂素，异补骨脂素，巴瓦钦，补骨脂素，异巴香素和补骨脂酚）色谱法。通过设计实验方法，优化了影响萃取效率的实验因素，包括萃取溶剂浓度，萃取溶剂体积和超声时间。在这种优化方法中，将中央复合设计作为二次模型进行了应用。在最佳条件下，良好的线性回归（r ² > 0.9999）并达到目标的回收率（96.8％–104％，RSD <3.73％）。此外，分析物的日内含量低于4.42％，分析物的日间精度低于2.08％。此提议的方法仅需要少量试剂（0.5 mL）和材料用量（20 mg）。因此，本研究应用了有效的方法来显示快速制备和测定s骨中五大洲的八种成分。